Roles of matricryptins (bioactive extracellular matrix fragments) in adipose tissue
基质隐蛋白(生物活性细胞外基质片段)在脂肪组织中的作用
基本信息
- 批准号:RGPIN-2020-06363
- 负责人:
- 金额:$ 2.62万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2020
- 资助国家:加拿大
- 起止时间:2020-01-01 至 2021-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Studying the instructive cues provided by the extracellular matrix (ECM) is crucial, and represents an emerging field for adipose tissue. Matricryptins are bioactive fragments generated by the proteolytic remodeling of the ECM. Importantly, these fragments exert intrinsic biological activities that can differ from those of their parent biomolecules. Matricryptins have been shown to regulate cell behavior, in particular for endothelial cells. We hypothesize that selected recombinant matricryptins can stimulate angiogenesis in adipose tissue while preserving the key metabolic functions of this tissue.
We will use a biomimetic model of adipose tissue engineered in vitro featuring a microvasculature of endothelial cell-derived capillary networks. Our method is based on the capacity of adipose-derived stromal/stem cells (ASCs) to produce and assemble an abundant endogenous ECM while differentiating into adipocytes. Our specific aims are to: Ia. Determine the impact of selected matricryptin supplementation on endothelial cells and the developing microvasculature in engineered adipose tissues. Ib. Assess if these matricryptins affect adipocyte functions and adipogenesis. II. Determine the endogenous profiles of expression and release of matricryptins in adipose tissues.
Aim Ia. Matricryptin candidates have been selected for their reported proangiogenic properties in specific contexts while their roles in adipose tissue are unknown. They will be supplemented into the culture media during adipose tissue formation in vitro and dose-response studies will be carried out. Detailed characterization of the capillary networks formed in matricryptin-enriched or control cultures will be performed by live monitoring as well as confocal microscopy. Aim Ib. The impact on lipolysis will be assessed by quantifying glycerol release by adipocytes upon isoproterenol stimulation. Adipokines with angiomodulatory actions will be quantified. The extent of differentiation of ASCs into adipocytes in presence of matricryptins will also be performed.
Aim II. We will determine the endogenous profiles of expression and release of matricryptins in adipose tissue in absence of exogenously added matricryptins. We will identify and quantify the major matricryptins present in human adipose tissue using quantitative mass spectrometry on samples of engineered and native adipose tissues.
Impact: The study of matricryptins in adipose tissues has been mostly focused so far on a single matricryptin, endotrophin. Our findings will reveal the capacity of other matricryptins to act as regulators of angiogenesis and/or adipogenesis in adipose tissue. This work will lead to a better understanding of the molecular mechanisms through which ECM components and their bioactive fragments can modulate the microenvironment and impact cell behavior during adipose tissue development and remodeling. Producing a map of matricryptin expression in adipose tissue will contribute novel basic knowledge.
研究细胞外基质(ECM)提供的指导性线索至关重要,并且代表了脂肪组织的新兴领域。基质蛋白是由ECM的蛋白水解重塑产生的生物活性片段。重要的是,这些片段发挥的内在生物活性可能不同于它们的母体生物分子。基质蛋白酶已被证明可以调节细胞行为,特别是内皮细胞。我们假设选择的重组基质蛋白可以刺激脂肪组织中的血管生成,同时保留该组织的关键代谢功能。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Fradette, Julie其他文献
Production of Tissue-Engineered Skin Substitutes for Clinical Applications: Elimination of Serum.
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10.3390/ijms241612537 - 发表时间:
2023-08-08 - 期刊:
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Doucet, Emilie J.;Cortez Ghio, Sergio;Barbier, Martin A.;Savard, Etienne;Magne, Brice;Safoine, Meryem;Larouche, Danielle;Fradette, Julie;Germain, Lucie - 通讯作者:
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Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells.
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10.1016/j.stemcr.2015.05.004 - 发表时间:
2015-06-09 - 期刊:
- 影响因子:5.9
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Talele, Nilesh P.;Fradette, Julie;Davies, John E.;Kapus, Andras;Hinz, Boris - 通讯作者:
Hinz, Boris
Harvesting the Potential of the Human Umbilical Cord: Isolation and Characterisation of Four Cell Types for Tissue Engineering Applications
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10.1159/000341254 - 发表时间:
2013-01-01 - 期刊:
- 影响因子:2.7
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Hayward, Cindy J.;Fradette, Julie;Auger, Francois A. - 通讯作者:
Auger, Francois A.
Creating capillary networks within human engineered tissues: Impact of adipocytes and their secretory products
- DOI:
10.1016/j.actbio.2014.09.044 - 发表时间:
2015-01-01 - 期刊:
- 影响因子:9.7
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Aubin, Kim;Vincent, Caroline;Fradette, Julie - 通讯作者:
Fradette, Julie
A Newly Developed Chemically Defined Serum-Free Medium Suitable for Human Primary Keratinocyte Culture and Tissue Engineering Applications.
- DOI:
10.3390/ijms24031821 - 发表时间:
2023-01-17 - 期刊:
- 影响因子:5.6
- 作者:
Ghio, Sergio Cortez;Barbier, Martin A.;Doucet, Emilie J.;Debbah, Imad;Safoine, Meryem;Le-Bel, Gaetan;Cartier, Andreanne;Jolibois, Emilie;Morissette, Amelie;Larouche, Danielle;Fradette, Julie;Guerin, Sylvain L.;Garnier, Alain;Germain, Lucie - 通讯作者:
Germain, Lucie
Fradette, Julie的其他文献
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{{ truncateString('Fradette, Julie', 18)}}的其他基金
Roles of matricryptins (bioactive extracellular matrix fragments) in adipose tissue
基质隐蛋白(生物活性细胞外基质片段)在脂肪组织中的作用
- 批准号:
RGPIN-2020-06363 - 财政年份:2022
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
Roles of matricryptins (bioactive extracellular matrix fragments) in adipose tissue
基质隐蛋白(生物活性细胞外基质片段)在脂肪组织中的作用
- 批准号:
RGPIN-2020-06363 - 财政年份:2021
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
Interactions cellules-matrice extracellulaire: impact sur les tissues reconstruits par génie tissulaire
细胞与细胞外基质的相互作用:对组织重建的影响
- 批准号:
326987-2011 - 财政年份:2015
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
Interactions cellules-matrice extracellulaire: impact sur les tissues reconstruits par génie tissulaire
细胞与细胞外基质的相互作用:对组织重建的影响
- 批准号:
326987-2011 - 财政年份:2014
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
Interactions cellules-matrice extracellulaire: impact sur les tissues reconstruits par génie tissulaire
细胞与细胞外基质的相互作用:对组织重建的影响
- 批准号:
326987-2011 - 财政年份:2013
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
Interactions cellules-matrice extracellulaire: impact sur les tissues reconstruits par génie tissulaire
细胞与细胞外基质的相互作用:对组织重建的影响
- 批准号:
326987-2011 - 财政年份:2012
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
Interactions cellules-matrice extracellulaire: impact sur les tissues reconstruits par génie tissulaire
细胞与细胞外基质的相互作用:对组织重建的影响
- 批准号:
326987-2011 - 财政年份:2011
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
Évaluation de l'établissement et de la croissance de la régénération forestière
林业监管机构和新月的评估
- 批准号:
400432-2010 - 财政年份:2010
- 资助金额:
$ 2.62万 - 项目类别:
University Undergraduate Student Research Awards
Régénération et croissance de l'éoinette noirce la pessière à mousse
鼠尾草慕斯的调理和面包
- 批准号:
394234-2010 - 财政年份:2010
- 资助金额:
$ 2.62万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Master's
Matrice extracellulaire et différenciation adipocytaire: analyse des interactions dans un contexte tridimensionnel in vitro
细胞外矩阵和脂肪细胞分化:分析体外三维环境中的相互作用
- 批准号:
326987-2006 - 财政年份:2010
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
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Discovery Grants Program - Individual
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