Developing a platform for rapid online DNA monitoring of microbial communities in wastewater treatment systems

开发废水处理系统中微生物群落快速在线 DNA 监测平台

• 批准号: 556792-2020
• 负责人: Ziels, Ryan
• 金额: $ 5.97万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=721627
• 关键词: Developing; platform; rapid; online; DNA

This proposed project is a partnership with Metro Vancouver to develop improved technologies for the detection and mitigation of problematic microorganisms in secondary treatment processes at their wastewater treatment plants (WWTPs), with a view to optimizing treatment performance, reliability, and ultimately environmental water quality. WWTPs provide the restorative function of the urban water cycle, and typically include primary treatment to remove particulate carbon materials, and secondary treatment to remove soluble organic compounds as well as nutrients like nitrogen and phosphorus. Secondary wastewater treatment is entirely dependent on microorganisms to degrade different forms of pollution present in wastewater, and to minimize impacts on receiving waters. However, certain microorganisms in secondary wastewater treatment can cause process instability and upsets due to foaming or poor-settling (bulking). This project will focus on developing protocols using next-generation portable DNA sequencing technology to rapidly profile wastewater microbial communities. Through active microbial monitoring using these emerging detection methods, it will be possible to gain early warning signals for when problematic microorganisms are growing, and develop strategies to tune secondary process operation accordingly to meet regulated effluent standards and maintain ecosystem health in Canada's environmental receiving waters. This project will support the training of the next-generation of environmental engineers by involving 4 graduate students in innovative solutions applying genomics, bioinformatics, and data science.

该拟议的项目是与温哥华大都会的合作伙伴关系，旨在开发改进的技术，用于在其废水处理厂（WWTPS）的二级治疗过程中检测和缓解有问题的微生物，以期优化治疗性能，可靠性，可靠性，最终环保水质。 WWTP提供了城市水周期的恢复功能，通常包括去除颗粒碳材料的主要治疗方法，以及去除可溶性有机化合物以及氮和磷等养分。次级废水处理完全取决于微生物，以降解废水中存在的不同形式的污染，并最大程度地减少对接收水的影响。但是，在次级废水处理中的某些微生物可能会导致过程不稳定和由于泡沫或不良的（散装）而引起的不稳定。该项目将着重于使用下一代便携式DNA测序技术来开发协议，以快速介绍废水微生物群落。通过使用这些新兴检测方法进行主动的微生物监测，有可能在有问题的微生物增长时获得预警信号，并制定相应地调整次要过程操作的策略，以满足受管制的废水标准并维持加拿大环保水域中的生态系统健康。该项目将通过参与使用基因组学，生物信息学和数据科学的创新解决方案，来支持对环境工程师下一代的培训。