Stress-induced flowering response to abiotic stimuli in sustainable bio-systems

可持续生物系统中应激诱导的开花对非生物刺激的反应

• 批准号: RGPIN-2019-04931
• 负责人: Gravel, Valérie
• 金额: $ 2.04万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=737955
• 关键词: Stress; induced; flowering; response; abiotic

Flowering in horticultural crops is the result of physiological, chemical and molecular changes. Regulation of these is done either autonomously (e.g. genotype) or through the effect of environmental factors (e.g. photoperiod or temperature). Flower bud induction remains a limiting factor in intensive and sustainable fruit production systems. Recently, stress-induced flowering has received increased attention as a potential way to manipulate fruit set and the overall quality of produced fruits due to the plant response to stress. Using Fragaria spp. as a model plant, my research program is based on the general hypothesis that fruit set and quality composition can be enhanced through manipulation of stress-induced flowering in day-neutral species. The long-term objective is to elucidate the biochemical and genetic mechanisms that elicit and regulate stress-induced flowering in Fragaria spp. and evaluate the subsequent impact on berry quality. My research team and I will first of all 1) characterize the physiological flowering response of day-neutral Fragaria spp. to individual and combined abiotic stimuli. We will then 2) elucidate the role of secondary metabolites as signal molecules regulating flower bud induction in Fragaria spp. in response to abiotic stimuli. Finally, we will 3) characterize the regulation of key genes involved in stress-induced flowering in Fragaria vesca. These objectives will be achieved through a well-balanced combination of classic phenotypic assessment of plant development and state-of-the-art strategies for transcriptomic and metabolite analysis (RNA-seq and HPLC). The knowledge generated by the three proposed objectives will provide a clear understanding of the regulation of stress-induced flowering in Fragaria spp. and will highlight the potential use of abiotic stimuli manipulation to enhance flowering and berry quality. This will benefit the Canadian strawberry industry by providing new practices to improve fruit production as well as consumers by increasing availability of locally-produced berries. My research answers a need to pursue discoveries in the field of sustainable development within the biofood industry to ensure long-term health benefits for consumers by promoting improved resource use efficiency in high-quality fruit production, which directly addresses one of the seven priority areas targeted by the Canadian government. My research program will also have a strong socio-economic impact through the unique multidisciplinary training platform for Ph.D. (2), M.Sc. (2) and undergraduate (5) students, allowing them to gain expertise and a complete skill set in a sector characterized by a high demand in trained scientists.

园艺作物的开花是生理、化学和分子变化的结果。这些调节是自主完成的（例如基因型）或通过环境因素的影响（例如光周期或温度）。花芽诱导仍然是集约化和可持续水果生产系统的限制因素。近年来，由于植物对胁迫的反应，胁迫诱导的开花作为操纵坐果和所生产果实的整体质量的潜在方式受到越来越多的关注。利用草莓属（Fragaria spp.）作为一种模式植物，我的研究计划是基于这样一个一般假设，即通过操纵白天中性物种的胁迫诱导开花可以提高坐果率和质量组成。长期的目标是阐明生化和遗传机制，引发和调节应力诱导开花草莓。并评估对浆果质量的后续影响。我和我的研究小组将首先1）描述日中性草莓的生理开花反应。单独和组合的非生物刺激。我们将2）阐明次生代谢产物作为信号分子调控草莓花芽诱导的作用。对非生物刺激的反应。最后，我们将3）表征参与胁迫诱导的草莓开花的关键基因的调控。这些目标将通过植物发育的经典表型评估与转录组学和代谢物分析（RNA-seq和HPLC）的最新策略的良好平衡组合来实现。这三个目标所产生的知识将提供一个清晰的理解，在草莓属植物的压力诱导开花的调节。并将突出非生物刺激操纵的潜在用途，以提高开花和浆果质量。这将有利于加拿大草莓产业，提供新的做法，以改善水果生产，以及消费者通过增加当地生产的浆果的可用性。我的研究回答了在生物食品行业内寻求可持续发展领域的发现的需要，以通过促进提高高品质水果生产的资源利用效率来确保消费者的长期健康利益，这直接解决了加拿大政府针对的七个优先领域之一。我的研究计划也将通过博士学位独特的多学科培训平台产生强大的社会经济影响。(2)、理学硕士（2）和本科生（5），使他们能够在对训练有素的科学家需求很高的行业中获得专业知识和完整的技能。