Stress-induced flowering response to abiotic stimuli in sustainable bio-systems

可持续生物系统中应激诱导的开花对非生物刺激的反应

• 批准号: RGPIN-2019-04931
• 负责人: Gravel, Valérie
• 金额: $ 2.04万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=737955
• 关键词: Stress; induced; flowering; response; abiotic

Flowering in horticultural crops is the result of physiological, chemical and molecular changes. Regulation of these is done either autonomously (e.g. genotype) or through the effect of environmental factors (e.g. photoperiod or temperature). Flower bud induction remains a limiting factor in intensive and sustainable fruit production systems. Recently, stress-induced flowering has received increased attention as a potential way to manipulate fruit set and the overall quality of produced fruits due to the plant response to stress. Using Fragaria spp. as a model plant, my research program is based on the general hypothesis that fruit set and quality composition can be enhanced through manipulation of stress-induced flowering in day-neutral species. The long-term objective is to elucidate the biochemical and genetic mechanisms that elicit and regulate stress-induced flowering in Fragaria spp. and evaluate the subsequent impact on berry quality. My research team and I will first of all 1) characterize the physiological flowering response of day-neutral Fragaria spp. to individual and combined abiotic stimuli. We will then 2) elucidate the role of secondary metabolites as signal molecules regulating flower bud induction in Fragaria spp. in response to abiotic stimuli. Finally, we will 3) characterize the regulation of key genes involved in stress-induced flowering in Fragaria vesca. These objectives will be achieved through a well-balanced combination of classic phenotypic assessment of plant development and state-of-the-art strategies for transcriptomic and metabolite analysis (RNA-seq and HPLC). The knowledge generated by the three proposed objectives will provide a clear understanding of the regulation of stress-induced flowering in Fragaria spp. and will highlight the potential use of abiotic stimuli manipulation to enhance flowering and berry quality. This will benefit the Canadian strawberry industry by providing new practices to improve fruit production as well as consumers by increasing availability of locally-produced berries. My research answers a need to pursue discoveries in the field of sustainable development within the biofood industry to ensure long-term health benefits for consumers by promoting improved resource use efficiency in high-quality fruit production, which directly addresses one of the seven priority areas targeted by the Canadian government. My research program will also have a strong socio-economic impact through the unique multidisciplinary training platform for Ph.D. (2), M.Sc. (2) and undergraduate (5) students, allowing them to gain expertise and a complete skill set in a sector characterized by a high demand in trained scientists.

园艺作物开花是生理、化学和分子变化的结果。对这些的调节是自主进行的(例如，基因)，或者是通过环境因素的影响(例如，光周期或温度)。在集约和可持续的水果生产系统中，花芽诱导仍然是一个限制因素。近年来，由于植物对逆境的反应，逆境诱导开花作为一种潜在的调控座果和果实整体品质的方式受到越来越多的关注。利用Fragaria spp.作为一种模式植物，我的研究计划基于这样一个普遍假设，即通过操纵胁迫诱导的日光中性物种的开花可以提高坐果率和品质组成。本研究的长期目标是阐明诱导和调控Fragaria spp.胁迫诱导开花的生化和遗传机制。并评估后续对浆果品质的影响。我和我的研究团队首先将1)描述中性日开花的生理反应。对于单独的和组合的非生物刺激。然后，我们将阐明次生代谢物作为信号分子在调节花芽诱导中的作用。对非生物刺激的反应。最后，我们将对胁迫诱导花期关键基因的调控进行研究。这些目标将通过对植物发育的经典表型评估和转录和代谢物分析的最新战略(RNA-SEQ和高效液相色谱)的良好平衡组合来实现。这三个拟议目标所产生的知识将使人们清楚地了解胁迫诱导的Fragaria spp.开花的调控。并将突出非生物刺激操纵的潜在用途，以提高开花和浆果质量。这将使加拿大草莓产业受益，因为它提供了新的做法，通过增加当地生产的浆果的供应来提高水果产量和消费者。我的研究满足了在生物食品行业内寻求可持续发展领域的发现的需求，以通过促进高质量水果生产中提高资源利用效率来确保消费者的长期健康利益，这直接涉及加拿大政府目标的七个优先领域之一。我的研究项目还将通过博士(2)、理科硕士(M.Sc)独特的多学科培训平台产生强大的社会经济影响。(2)和本科(5)学生，使他们能够在一个对训练有素的科学家需求很高的部门获得专业知识和完整的技能。