Electrochemical biosensors for point-of-care diagnostics

用于即时诊断的电化学生物传感器

• 批准号: RGPIN-2020-05884
• 负责人: Merle, Geraldine
• 金额: $ 2.4万
• 依托单位: École Polytechnique de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=740892
• 关键词: Electrochemical; biosensors; point; care; diagnostics

Current technologies for in vitro and clinical assessment are costly, complicated, labour intensive, and time-consuming. Additionally, since molecular diagnostic tests and instrumentation are complex, they require highly knowledgeable and expert scientists to establish benchmarks. And so, they cannot provide real time decision support. The important unmet need (the primary driver behind my research program) is to develop easy-to-use, quick, and affordable electrochemical analytical solutions that can be easily implemented in either a clinical environment or the remote areas.  Recent literature has indicated that overexpression of metalloproteins - proteins which contain metal atoms or clusters have been associated with human health and diseases. Based on the overall hypothesis that some diseases overexpress specific metalloproteins, and that their metals can interact directly with an electrode surface and specifically display reversible electrochemistry, I aim to electrochemically detect those biomarkers with innovative sensing devices to assist clinicians in taking decisions. As an expert in the synthesis of functional nanomaterials, and electroconductive substrate, here, over the next 5 years, my research objectives will be to focus on 1) designing and building a multifunctional platform of advanced conductive materials and functional interface for the electrochemical detection and monitoring of metalloproteins and pathogens for health and environmental analysis and management, 2) modifying traditional electrochemical cells into tools adapted to in vivo environment, and 3) evaluating these innovative implantable electrochemical devices to aid in disease detection and management, and monitoring healing.  My research program provides excellent training environment for HQP due to it interdisciplinary nature involving materials science, biomedical engineering, electrochemistry. The training is further enriched by the benefit of testing our electrochemical devices in vivo and in clinical environment with real end-product users.  This research program will develop medicaltech platforms technology will not only be important for both academia and industry. Further, they aim to reduce the economic burden to health care services by providing more accurate and more immediate analyses of disease and normal physiology.

目前用于体外和临床评估的技术昂贵、复杂、劳动密集型和耗时。此外，由于分子诊断测试和仪器设备很复杂，它们需要具有高度知识和专业知识的科学家来建立基准。因此，他们不能提供实时决策支持。尚未满足的重要需求(我的研究计划背后的主要驱动力)是开发易于使用、快速和负担得起的电化学分析解决方案，这些解决方案可以在临床环境或偏远地区轻松实施。最近的文献表明，金属蛋白的过度表达与人类健康和疾病有关。金属蛋白是一种含有金属原子或簇的蛋白质。基于一些疾病过度表达特定金属蛋白的总体假设，以及它们的金属可以直接与电极表面相互作用并特异地显示可逆电化学，我的目标是使用创新的传感设备对这些生物标志物进行电化学检测，以帮助临床医生做出决定。作为功能纳米材料和导电衬底的合成专家，在未来5年内，我的研究目标将集中在：1)设计和构建先进导电材料的多功能平台和功能接口，用于金属蛋白和病原体的电化学检测和监测，用于健康和环境分析与管理；2)将传统的电化学细胞改造成适应体内环境的工具；3)评估这些创新的植入式电化学设备，以帮助疾病检测和管理以及监测愈合。由于我的研究计划涉及材料科学、生物医学工程、电化学等多学科，为HQP提供了良好的培训环境。通过在活体和临床环境中与真正的最终产品用户一起测试我们的电化学设备，进一步丰富了培训内容。这项研究计划将开发医疗技术平台，技术不仅对学术界和产业界都很重要。此外，它们的目标是通过提供更准确和更及时的疾病和正常生理分析来减轻卫生保健服务的经济负担。