Bioinspired mechanogating biosensors for real-time biodetection

项目摘要 电子生物传感器在医学诊断中发挥着越来越重要的作用。早些时候 需要检测各种生物标志物，以便为预防和治疗提供及时的诊断。 疾病。然而，目前的电子生物传感器在生物检测方面受到限制，往往被认为是非特异性的。 体内溶液中复杂离子环境的干扰。具体地说，指控筛查具有 防止了场效应式生物传感器在生理环境中的实时生物检测。我们打算把 提出并验证了一种新的生物传感器概念，它可以不受非特异性电荷干扰，并提供 针对特定生物检测的通用解决方案。我们还打算将该生物传感器应用于扁虱的早期检测- 传染病对公众健康构成严重威胁，但缺乏及早发现 及时救治。受生物细胞器中机械转导的启发，我们将采用一种 “机械感应”感应机构，它是正交的，因此对电荷干扰有弹性。具体来说， 我们建议设计一种基于暴露在分析物流动中的悬浮纳米晶体管的生物传感器；结合 生物分子有望增加纳米晶体管的有效横截面积，因此 流体流动的阻力；感应应变将通过压阻引起电导变化 效果。 为了实现这些目标，在目标1中，我们将组装和集成高度悬浮的纳米晶体管作为 为拟议的传感机制专门设计的生物传感器。在目标2中，我们将评估和验证 生物传感器在模拟生理环境的高离子强度下的功能和性能。在《目标3》中， 我们将实施生物传感器，选择性地检测硬蜱传播疾病的病原体。如果成功， 该生物传感器将为改善/及时治疗硬虱传播的疾病提供实用的解决方案。这个 这项研究有望创造一种新的生物传感器，它将超越场效应的能力 生物传感器，实现生理环境中的通用生物检测，引领先进的生物医学 用于多功能护理点诊断的设备。

Project Summary Electronic biosensors have been playing increasingly important roles in medical diagnostics. Early detections of various biomarkers are desired to provide timely diagnosis for the prevention and treatment of diseases. However, current electronic biosensors are limited in biodetection, often attributed to nonspecific interferences from a complicated ionic environment in bodily solutions. Specifically, charge screening has prevented field-effect biosensors from real-time biodetection in physiological environment. We intend to bring in and validate a new biosensor concept, which can be free of nonspecific charge interference and provide generic solution to the specific biodetection. We also intend to apply the biosensor in the early detection of tick- borne diseases, which have imposed serious threat to public health but lacked means in early detection for timely treatment. Inspired by the mechanotransduction in biological organelles, we will employ a `mechanogating' sensing mechanism that is orthogonal and hence resilient to charge interference. Specifically, we propose to design a biosensor based on a suspended nanotransistor exposed to analyte flow; the binding biomolecules are expected to increase the effective cross-sectional area of the nanotransistor and hence the drag force by the fluid flow; the induced strain will lead to a conductance change through the piezoresistance effect. To realize the goals, in Aim 1 we will assembly and integrate highly suspended nanotransistors as the biosensors specifically designed for the proposed sensing mechanism. In Aim 2, we will evaluate and verify the biosensor function and performance in high ionic strength mimicking the physiological environment. In Aim 3, we will implement the biosensor for the selective detection of pathogens of tick-borne diseases. If successful, the biosensor will provide a practical solution for improved/timely treatment in tick-borne diseases. The research is expected to create a new class of biosensors, which will transcend the inability of field-effect biosensors and realize generic biodetection in physiological environment, leading to advanced biomedical devices for versatile point-of-care diagnostics.