Structure-function relationships in biomaterials for tissue engineering applications

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To contribute to strengthen Canada's leadership in biomedical technologies, I propose a research program aimed at addressing both long-standing problems and current challenges in tissue engineering by developing biomaterials-driven solutions. In particular, I will investigate still unexplored structure-function relationships governing cell behaviour at interfaces and in 3D matrices to generate the fundamental new knowledge required to instruct the design of more effective biomaterials for applications in bone, gut and uterine tissue engineering. Specifically, over the course of the next five years, I propose a new experimental approach to study interfacial phenomena with an in vitro cell culture model that mimics key aspects of bone micro-physiology in order to explain the mechanisms that control how cells response to nanopatterns with variable geometry and disorder. Results from this research will establish new in vitro practices and predictive models for the accurate assessment of the osteonductive potential of nanopatterned titanium surfaces for the biomedical implants market. In addition, I will develop and optimize hydrogels based on their in vitro ability to promote relevant functions of enteric neurons, addressing the current lack of systematic work focus on effective methods to support enteric neuronal progenitor differentiation and restoration of gut innervation. This research will lead to the creation of both injectable cell-free scaffolds to directly restore innervation in the gut and of tunable matrices for the encapsulation and delivery of enteric neuron progenitors for stem-cell therapies. Lastly, I will investigate and screen hydrogels based on their ability to simultaneously promote endometrial cell colonization and invasion. The goal is the development of novel uterine patches capable of inducing repair of uterine scars and injuries while, at the same time, thickening the viable volume of tissue at the site of the scar to provide a more favorable environment for placentation, ultimately favouring a positive outcome of pregnancy. Ultimately, the proposed research is poised to create profound transformations in the ways biomedical strategies and technologies are designed and applied: cost-effective, biomaterials-driven alternatives will ensure continued growth of Canada's leadership in biomedical research and industrial innovation.