Development of 3D culture systems for primary neuronal cells**

原代神经元细胞 3D 培养系统的开发**

• 批准号: 536354-2018
• 负责人: Variola, Fabio
• 金额: $ 1.82万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=667634
• 关键词: Development; 3D; culture; systems; primary

In vitro practices must overcome important challenges to compare favourably with in vivo human studies. The poor prediction and limited applicability of current in vitro assays and strategies can be explained by the fact that standard approaches do not enable recapitulation of the complexity of human tissues and physiological functions. In the case of existing neuronal cell culture systems, a major downside is that the 2D cell environment unrealistically flattens soma and growth cones, limits axon-dendrite outgrowth, and lacks many other constituents of a realistic in vivo environment.**The Engage program will permit development of 3D culture-based methods for neuronal cells. Results from this study are poised to build the scientific foundation that will ultimately allow our industrial partner, QBM Cell Science, to implement 3D cultures in their R&D protocols, allowing the company to transition to more physiologically accurate cell culture systems.**

体外实践必须克服重要的挑战，以比较有利的在体内的人的研究。目前体外检测和策略的预测能力差，适用性有限，这可以通过标准方法不能重现人体组织和生理功能的复杂性这一事实来解释。在现有的神经细胞培养系统中，一个主要的缺点是二维细胞环境不切实际地使体细胞和生长锥变平，限制了轴突树突的生长，并且缺乏现实体内环境的许多其他成分。** Engage项目将允许开发基于3D培养的神经细胞方法。这项研究的结果将奠定科学基础，最终使我们的工业合作伙伴QBM Cell Science能够在他们的研发方案中实施3D培养，从而使公司能够过渡到生理上更准确的细胞培养系统