Automated high resolution morphological and behavioural analysis system

自动化高分辨率形态和行为分析系统

• 批准号: RTI-2023-00487
• 负责人: Patten, Kessen(Shunmoogum)
• 金额: $ 10.91万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=743852
• 关键词: Automated; resolution; morphological; behavioural; analysis

A multidisciplinary team of researchers with expertise in studying development, neurosciences physiology, toxicology, pharmacology, virology and behaviour in zebrafish is requesting funds for the purchase of an automated high-resolution video tracking and analysis system. Specifically, the requested state-of-the-art video-tracking system (Ramona MCAM) will be used to evaluate zebrafish morphology and behaviour at high resolution and high throughput. This innovative system will enable fast, unbiased, high throughput, accurate, and consistent video-aided measurements of a broad range of morphological and behavioural parameters to address both fundamental and applied questions in neurobiology, animal physiology, toxicology, pharmacology and virology. Specifically, the requested equipment will allow the applicants and their team to: 1) investigate molecular mechanisms in neuronal development in zebrafish and 2) investigate the effects of virus infection, biomaterials and endocrine disruptors on the development, neurodevelopment, physiology and behaviour of zebrafish larvae. Our HQP will be provided with an invaluable opportunity for the cutting-edge training needed for their respective research areas as well as in high-resolution imaging and complex behavioural data analysis. They will be highly sought after by academia, government, or industry research departments as research using zebrafish becomes more significant for biomedical modelling of disease, drug screening, and toxicological studies.

一个由研究发育、神经科学生理学、毒理学、药理学、病毒学和斑马鱼行为的多学科研究人员组成的多学科团队正在申请资金，用于购买自动高分辨率视频跟踪和分析系统。具体地说，所要求的最先进的视频跟踪系统(Ramona MCAM)将用于在高分辨率和高吞吐量下评估斑马鱼的形态和行为。这一创新系统将实现对广泛的形态和行为参数的快速、无偏、高通量、准确和一致的视频辅助测量，以解决神经生物学、动物生理学、毒理学、药理学和病毒学中的基本和应用问题。具体地说，所要求的设备将使申请者和他们的团队能够：1)研究斑马鱼神经元发育的分子机制；2)调查病毒感染、生物材料和内分泌干扰物对斑马鱼幼体发育、神经发育、生理和行为的影响。我们的HQP将为其各自的研究领域以及高分辨率成像和复杂行为数据分析所需的尖端培训提供宝贵的机会。随着使用斑马鱼的研究在疾病的生物医学模型、药物筛选和毒理学研究中变得更加重要，它们将受到学术界、政府或行业研究部门的高度追捧。