Molecular Mechanisms of Marine Polysaccharide Depolymerization and Metabolism

海洋多糖解聚和代谢的分子机制

• 批准号: RGPIN-2019-03985
• 负责人: Boraston, Alisdair
• 金额: $ 5.03万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=744210
• 关键词: Molecular; Mechanisms; Marine; Polysaccharide; Depolymerization

Ocean plant biomass [i.e. microalgae and macroalgae (seaweed)] is estimated to be only ~1/200 that of terrestrial plant biomass. Indeed, most of the carbon incorporated by photosynthetic processes is in land plants. However, the annual turnover rate (total mass per time) of photosynthetically fixed carbon in the oceans is roughly equal to that on land, indicating a highly dynamic process with a normalized rate of recycling that is hundreds of times greater than on land. In both the marine and land environments this carbon is locked primarily in the form of sugar chains called polysaccharides. The microbial degradation of terrestrial plant polysaccharides, cellulose being the major example, has been intensely studied for over 50 years and it is therefore relatively well understood; the same is not true of marine algal polysaccharides. The polysaccharides present in seaweed comprise a group of complex sugars that are chemically different from those of terrestrial plants. The vision that drives this research program, therefore, is to identify and define the largely unexamined molecular features of the unique metabolic systems that enable bacteria to metabolize marine polysaccharides. Determining how photosynthetically fixed carbon is recycled in our oceans is fundamental to the basic goal of understanding the global carbon cycle. However, in our world of climate change, the promises of renewable resources, carbon neutral footprints, and alternative fuel sources are increasingly attractive. Marine algae are an extremely appealing renewable resource with a high carbohydrate content. The proposed research program is generating a knowledge platform that can be leveraged to design strategies for the conversion of this biomass into fermentable sugars, which could then be converted into biofuels or other useful bioproducts. Additionally, many marine polysaccharides are used in foods, cosmetics, agriculture, and potential medical therapeutics. This research is providing a new set of tools to enzymatically modify these polymers to potentially control their properties in these applications. Finally, this work is also providing novel insights into the metabolic capabilities of the bacteria that inhabit human and animal guts where they aid digestion of dietary marine polysaccharides. This innovative research program, which is the only one of its kind in Canada, has the potential to transform a field that has a remarkably wide impact.

海洋植物生物量[即微藻和大型藻类（海藻）]估计仅为陆地植物生物量的约1/200。事实上，光合作用过程中吸收的大部分碳都存在于陆地植物中。然而，海洋中光合作用固定碳的年周转率（每次总质量）与陆地上大致相等，表明这是一个高度动态的过程，其正常化再循环率是陆地的数百倍。在海洋和陆地环境中，这种碳主要以称为多糖的糖链的形式被锁定。陆地植物多糖的微生物降解，纤维素是主要的例子，已经被深入研究了50多年，因此它是相对较好的理解;海藻多糖的情况并非如此。海藻中存在的多糖包含一组化学上不同于陆生植物的复合糖。因此，推动这项研究计划的愿景是识别和定义使细菌能够代谢海洋多糖的独特代谢系统的基本上未经检查的分子特征。确定光合作用固定的碳如何在我们的海洋中循环，对于理解全球碳循环的基本目标至关重要。然而，在我们的气候变化世界中，可再生资源、碳中和足迹和替代燃料来源的前景越来越有吸引力。海藻是一种非常有吸引力的可再生资源，具有高碳水化合物含量。拟议的研究计划正在生成一个知识平台，可以利用该平台设计将生物质转化为可发酵糖的策略，然后将其转化为生物燃料或其他有用的生物产品。此外，许多海洋多糖用于食品，化妆品，农业和潜在的医疗。这项研究提供了一套新的工具来酶促修饰这些聚合物，以潜在地控制它们在这些应用中的特性。最后，这项工作还为栖息在人类和动物肠道中的细菌的代谢能力提供了新的见解，这些细菌有助于消化膳食海洋多糖。这个创新的研究项目是加拿大唯一的一个，有可能改变一个具有广泛影响的领域。