Hypoxic incubators to conduct studies in low oxygen environments

低氧培养箱可在低氧环境下进行研究

基本信息

  • 批准号:
    RTI-2023-00502
  • 负责人:
  • 金额:
    $ 2.34万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Research Tools and Instruments
  • 财政年份:
    2022
  • 资助国家:
    加拿大
  • 起止时间:
    2022-01-01 至 2023-12-31
  • 项目状态:
    已结题

项目摘要

One of the challenges of experimentation with cells under low oxygen (hypoxic) conditions is the return to atmospheric (21%) oxygen after cell treatment. Cellular changes can occur WITHIN 5 MINUTES of removal of cells from low (hypoxic or complete absence of oxygen (anoxic)) to atmospheric oxygen environments. Research requiring hypoxic conditions includes studies on cell stress response, inflammation, cancer biology, stem cell development and oxygen sensing by cells. Our work is impossible without this equipment. Currently we are relying upon triple gas (N2-O2-CO2) incubators to generate hypoxic conditions. However, these incubators are now 20 years old and are failing, having reached beyond their expected lifespan (which is 10 years). As we use them on a daily basis, our out-dated triple gas incubators are filled to capacity. These chambers operate under constant threat of failure. Over the past year, shut downs for repairs (replacement of O2 and CO2 sensors, leakage of the water jacket, leakage in internal tubing carrying gases, failure of controlling motherboards) has disrupted the experiments of 10 Undergraduate Students, 10 Graduate Students and 1 Postdoctoral trainees in the Willmore and Biggar labs. Set-backs like this waste precious time and cause enormous stress by interfering with data acquisition by students needed for publications, theses, and completion of undergraduate thesis projects. Things are reaching a breaking point. If these are not replaced with a better system within the next year, then our core research program (which relies on the generation of hypoxic environments) will not be able to continue. The projects of the trainees of the labs dependent upon this infrastructure will be greatly hindered by not having access to it. The equipment requested is two HeracellT 150i Tri-Gas Incubators. The proposed incubators would allow for controlled environments during cell manipulation and ensure that hypoxic conditions are maintained throughout the experiment. The hypoxic incubators will enable our lab (as well as collaborating labs) to maintain low oxygen conditions upon cell manipulation and harvest, as well as have programmed oxygen conditions (such as graded hypoxia or preconditioning hypoxia exposures) over time. There are no similar units at Carleton University and only a few within the Ottawa region that are inaccessible to the researchers and used for studies with bacteria and not mammalian cells. The hypoxic incubators are therefore CRITICAL for the continuation of the research programs of the Willmore and Biggar labs, as well as collaborating labs, that are dependent upon the generation of low oxygen environments.
在低氧(低氧)条件下进行细胞实验的挑战之一是在细胞处理后返回到大气(21%)氧。细胞从低氧(低氧或完全无氧(缺氧))环境移至大气氧气环境后5分钟内可发生细胞变化。需要低氧条件的研究包括细胞应激反应、炎症、癌症生物学、干细胞发育和细胞氧气感应的研究。没有这台设备,我们的工作是不可能的。目前我们依靠三重气体(N_2-O_2-CO_2)孵化器来产生低氧条件。然而,这些孵化器现在已经有20年的历史,而且正在失败,已经超过了它们的预期寿命(即10年)。由于我们每天都在使用它们,我们过时的三重气体孵化器已经满了。这些商会在持续不断的失败威胁下运作。在过去的一年里,停机维修(更换O2和CO2传感器、水套泄漏、内部输气管道泄漏、主板控制故障)扰乱了威尔莫尔和比格实验室的10名本科生、10名研究生和1名博士后的实验。像这样的挫折浪费了宝贵的时间,并造成了巨大的压力,因为干扰了学生为出版、论文和完成本科论文项目所需的数据获取。一切都快到临界点了。如果明年不能用更好的系统取代这些系统,那么我们的核心研究计划(依赖于低氧环境的产生)将无法继续下去。依赖于这一基础设施的实验室受训人员的项目将因无法使用而受到极大阻碍。所要求的设备是两个HeracellT 150i Tri-Gas孵化器。拟议的孵化器将允许在细胞操作期间控制环境,并确保在整个实验过程中保持低氧条件。低氧培养箱将使我们的实验室(以及合作实验室)在细胞操作和收获时保持低氧条件,并随着时间的推移编程氧气条件(如分级低氧或预适应低氧暴露)。卡尔顿大学没有类似的单位,渥太华地区只有几个单位是研究人员无法接触到的,用于细菌而不是哺乳动物细胞的研究。因此,低氧孵化器对于威尔莫尔和比格实验室以及合作实验室的研究计划的继续至关重要,这些实验室依赖于低氧环境的产生。

项目成果

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Willmore, William其他文献

Willmore, William的其他文献

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{{ truncateString('Willmore, William', 18)}}的其他基金

Signaling Cross-talk Between Endoplasmic Reticulum And Oxidative Stresses
内质网和氧化应激之间的信号串扰
  • 批准号:
    RGPIN-2017-06414
  • 财政年份:
    2022
  • 资助金额:
    $ 2.34万
  • 项目类别:
    Discovery Grants Program - Individual
Signaling Cross-talk Between Endoplasmic Reticulum And Oxidative Stresses
内质网和氧化应激之间的信号串扰
  • 批准号:
    RGPIN-2017-06414
  • 财政年份:
    2021
  • 资助金额:
    $ 2.34万
  • 项目类别:
    Discovery Grants Program - Individual
Signaling Cross-talk Between Endoplasmic Reticulum And Oxidative Stresses
内质网和氧化应激之间的信号串扰
  • 批准号:
    RGPIN-2017-06414
  • 财政年份:
    2020
  • 资助金额:
    $ 2.34万
  • 项目类别:
    Discovery Grants Program - Individual
Signaling Cross-talk Between Endoplasmic Reticulum And Oxidative Stresses
内质网和氧化应激之间的信号串扰
  • 批准号:
    RGPIN-2017-06414
  • 财政年份:
    2019
  • 资助金额:
    $ 2.34万
  • 项目类别:
    Discovery Grants Program - Individual
Hypoxic workstation to conduct studies in low oxygen environments
低氧工作站可在低氧环境下进行研究
  • 批准号:
    RTI-2019-00935
  • 财政年份:
    2018
  • 资助金额:
    $ 2.34万
  • 项目类别:
    Research Tools and Instruments
Signaling Cross-talk Between Endoplasmic Reticulum And Oxidative Stresses
内质网和氧化应激之间的信号串扰
  • 批准号:
    RGPIN-2017-06414
  • 财政年份:
    2018
  • 资助金额:
    $ 2.34万
  • 项目类别:
    Discovery Grants Program - Individual
Signaling Cross-talk Between Endoplasmic Reticulum And Oxidative Stresses
内质网和氧化应激之间的信号串扰
  • 批准号:
    RGPIN-2017-06414
  • 财政年份:
    2017
  • 资助金额:
    $ 2.34万
  • 项目类别:
    Discovery Grants Program - Individual
"Mitochondrial biogenesis and the decline of hypoxia, oxidative stress and toxin tolerance with age"
“线粒体生物发生以及缺氧、氧化应激和毒素耐受性随着年龄的增长而下降”
  • 批准号:
    250649-2012
  • 财政年份:
    2016
  • 资助金额:
    $ 2.34万
  • 项目类别:
    Discovery Grants Program - Individual
"Mitochondrial biogenesis and the decline of hypoxia, oxidative stress and toxin tolerance with age"
“线粒体生物发生以及缺氧、氧化应激和毒素耐受性随着年龄的增长而下降”
  • 批准号:
    250649-2012
  • 财政年份:
    2015
  • 资助金额:
    $ 2.34万
  • 项目类别:
    Discovery Grants Program - Individual
"Mitochondrial biogenesis and the decline of hypoxia, oxidative stress and toxin tolerance with age"
“线粒体生物发生以及缺氧、氧化应激和毒素耐受性随着年龄的增长而下降”
  • 批准号:
    250649-2012
  • 财政年份:
    2014
  • 资助金额:
    $ 2.34万
  • 项目类别:
    Discovery Grants Program - Individual

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1/2 药物开发和能力建设:UCR/CoH-CCC 合作伙伴关系
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  • 财政年份:
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