Development of next generation of breath collection systems

开发下一代呼吸收集系统

• 批准号: RGPIN-2021-03735
• 负责人: Hill, Jane
• 金额: $ 2.84万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=746773
• 关键词: Development; next; generation; breath; collection

Non-invasive, hand-held diagnostics inspire this Discovery Grant application. My vision is to develop breath test science to the point of routine application. To that end, we conduct research at multiple levels, from the systematic creation of robust sampling and data processing methodologies to diagnostic device innovation to the evaluation of markers and devices in human and animal clinical trials. Our primary application areas are in the respiratory infection detection area, where we have developed samplers for humans and animals, created the first few generations of data processing approaches, innovated around analytical instrumentation, discovered biomarkers for many common pathogens, generated insights into the origins of the biomarkers, and are conducting clinical validation studies. The focus of this proposal is the development of next generation systems for breath science. First, we will (1) validate next-generation systems for breath science, specifically developing a field-first core breath index for human as well as critical lab animals (mice and non-human primates). This list can be used to evaluate breath science innovations (e.g., evaluation of new technology), harmonizing data between research groups, baseline variance measurements in diseased animals, and so on. We will also test and validate low-flow, easy-to-integrate breath samplers for use in laboratory or clinical applications for humans and animals. These devices will produce reliable breath samples for biomarker discovery and validation. The will have novel sample cartridges that allow for direct integration into analytical instrumentation. Second, we will (2) develop micro-analytical systems for breath analysis. We will test and validate pre-microconcentrators for micro-analytical devices. Here, we will focus on the optimization of adsorption matrices, with a focus on trace molecule retention and release as well as develop a methodology for purging water from the device. We will also focus on effective integration of the pre-microconcentrator into two micro-analytical systems. The resulting micro-analytical systems will then be ready for field testing in human, animal, or other research scenarios. 2 PhD, 2 MS, and 4-6 undergraduates will be trained in data science, analytical chemistry, instrumentation engineering, and breath science on this award.

非侵入性的手持式诊断激发了这项发现补助金申请。我的愿景是将呼吸测试科学发展到常规应用的程度。为此，我们在多个层面进行研究，从系统创建强大的采样和数据处理方法，到诊断设备创新，再到人类和动物临床试验中的标记物和设备评估。我们的主要应用领域是呼吸道感染检测领域，我们开发了人类和动物的采样器，创建了前几代数据处理方法，围绕分析仪器进行了创新，发现了许多常见病原体的生物标志物，深入了解了生物标志物的起源，并正在进行临床验证研究。这项建议的重点是为呼吸科学开发下一代系统。 首先，我们将（1）验证呼吸科学的下一代系统，特别是为人类以及关键实验室动物（小鼠和非人类灵长类动物）开发一个领域第一的核心呼吸指数。此列表可用于评估呼吸科学创新（例如，我们还将测试和验证用于人类和动物实验室或临床应用的低流量、易于集成的呼吸采样器。这些设备将产生可靠的呼吸样本，用于生物标志物的发现和验证。将有新的样品盒，允许直接集成到分析仪器。 其次，我们将（2）开发用于呼吸分析的微型分析系统。我们将测试和验证微型分析设备的预浓缩器。在这里，我们将重点关注吸附基质的优化，重点关注痕量分子的保留和释放，以及开发一种从设备中清除水的方法。我们还将专注于将预微浓缩器有效地集成到两个微分析系统中。由此产生的微分析系统将准备在人类，动物或其他研究场景中进行现场测试。 2名博士，2名硕士和4-6名本科生将在数据科学，分析化学，仪器工程和呼吸科学方面接受培训。