Microarchitecture for advanced polymeric materials

先进聚合物材料的微结构

• 批准号: CRC-2021-00196
• 负责人: Hwang, DaeKun
• 金额: $ 5.46万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Canada Research Chairs
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=747037
• 关键词: Microarchitecture; advanced; polymeric; materials

Diagnostics are an essential part of the healthcare system. Early and accurate detection of disease indicators is vital for reducing transmission, prophylaxis, and successful treatments. One of the ways to improve diagnostics is faster and improved testing, which can support large population screening and improve healthcare responsiveness. There is a growing demand for simultaneous (parallel) multiplexed immunoassays in the same sample, to allow testing for multiple diseases concurrently. Building upon his previous work, Dr. Dae Kun Hwang will develop and design innovative biomedical platforms utilizing functionalized smart polymeric materials: i) a hydrogel-membrane microfluidic chip for a highly rapid multiplex immunoassay, and ii) a sieved-microwell microfluidic chip for cell capture and single cell analysis.Dr. Hwang's invention of a highly rapid and simultaneous multiplexed immunoassay platform, based on a hydrogel membrane, will detect and quantify various biomarkers concurrently in the same sample. Microfluidics also require smaller samples, are cheaper, and easier to automate. These advantages will greatly reduce testing and detection time. Dr. Hwang has recently developed an unprecedented microfluidic fabrication method which will enable this testing.Microwells have emerged as a potential candidate for a wide range of applications, including single cell analysis, as they can capture a well-defined number of cells for study. Individual cells captured in microwells can be investigated for environmental responses, leading to discoveries of biochemical functions and cellular mechanisms, and can form the basis for drug discovery, diagnostics, cancer research, and regenerative medicine. A well-defined number of cancer cells trapped in microwells can be used as an effective model for drug testing.Dr. Hwang has developed a device for efficient cell trapping by crossflow, which provides great control not only by the number of cells but also shape, unseen in other microwell devices. This can be also effective for circulating tumor cell (CTC) detection. Dr. Hwang is developing scalable fabrication to make the CTC detection platform both low-cost and high throughput. He is also designing a microwell platform to facilitate uniformly-sized tumour spheroid capture, which is essential in drug testing. Dr. Hwang's innovative and versatile biomedical tools will allow him to improve the quality of healthcare and wellbeing on a large scale.

诊断是医疗保健系统的重要组成部分。早期和准确发现疾病指标对于减少传播、预防和成功治疗至关重要。改进诊断的方法之一是更快和改进的测试，这可以支持大规模人群筛查并提高医疗响应能力。在同一样品中同时（平行）多重免疫分析的需求日益增长，以便同时检测多种疾病。在他之前工作的基础上，Hwang博士将开发和设计利用功能化智能聚合物材料的创新生物医学平台：i)用于高速多重免疫分析的水凝胶膜微流控芯片，ii)用于细胞捕获和单细胞分析的筛孔微流控芯片。Hwang发明了一种基于水凝胶膜的高速、同步多路免疫分析平台，将在同一样品中同时检测和量化各种生物标志物。微流体也需要更小的样品，更便宜，更容易自动化。这些优点将大大减少测试和检测时间。黄博士最近开发了一种前所未有的微流体制造方法，将使这项测试成为可能。微孔已经成为广泛应用的潜在候选者，包括单细胞分析，因为它们可以捕获明确数量的细胞进行研究。在微孔中捕获的单个细胞可以研究环境反应，从而发现生化功能和细胞机制，并可以形成药物发现、诊断、癌症研究和再生医学的基础。被困在微孔中的明确数量的癌细胞可以作为药物测试的有效模型。黄禹锡开发出了一种通过交叉流动有效捕获细胞的装置，这种装置不仅可以控制细胞的数量，还可以控制细胞的形状，这在其他微孔装置中是看不到的。这对于循环肿瘤细胞（CTC）的检测也是有效的。Hwang博士正在开发可扩展的制造技术，使CTC检测平台既低成本又高通量。他还在设计一个微孔平台，以促进均匀大小的肿瘤球体捕获，这在药物测试中是必不可少的。黄博士的创新和多功能生物医学工具将使他能够大规模提高医疗保健和福祉的质量。