INTEGRATION OF BIOMECHANICAL LOADING IN 3D TISSUE GRAFTS AND NOVEL MATERIALS DEVELOPMENT

3D 组织移植和新材料开发中生物力学负载的集成

• 批准号: RTI-2023-00366
• 负责人: Kishen, Anil
• 金额: $ 10.93万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=750707
• 关键词: INTEGRATION; BIOMECHANICAL; LOADING; 3D; TISSUE

The universal mechanical testing system (Instron) as we know today has been an indispensable equipment across many fields of research. Since the first incarnation of Instron - Universal Testing Machines with strain gauge load cells in 1946 it has become the workhorse for biomechanical research providing the ability to inspect and measure biomechanical response forces, opening up a staggering number of new areas for researchers to explore and allowing them to better understand and quantify stress-strain relationship in biological and engineered systems. Among the impacts was the discovery of the relationship between structure and function in biological system and development of biomimetic biomaterials. Recent advances in these systems and approaches has enabled researchers to visualize and measure dynamic events. The requested Universal testing system will replace our decommissioned universal testing system, with the ElectroPulsr E1000. The decommission universal testing machine is an Instron 4301. It operational in the lab for more than 30 years and had to be decommissioned due to an irreplaceable part failure. The requested system will be compatible with our existing fixtures that have been designed over the last two decades for mounting samples appropriately to perform fatigue testing reliably on various biomaterials, especially dental implants. This system will be located adjacent to the Class II biosafety labs and integrated with the advanced microscopy/imaging facilities at the Faculty of Dentistry. This will be a unique facility to conduct biomechanical studies on in-vitro tissue grafts and in-situ hard tissues. The system will be supported by our current user base. This facility will allow the PIs and co-applicants to study static and dynamic biomechanical events to gain an improved understanding of hard tissues and synthetic biomaterials. Dr. Kishen investigates three-dimensional tissue grafts and dentin tissue, Dr. Bozec studies collagen structural and biomechanical properties, Dr. Changoor studies cartilage biomechanical in Osteoarthritis, Dr. Manolson investigates bone biomechanics in Osteoporosis, Dr. Santerre studies free radical generated polyurethane chemistry, which affords the chemical diversity of block copolymers, the ability to phase separate to form intermolecular domain structures to yield physical cues that influence cell function, to drive a program on innovative new polymeric biomaterials development. Dr. Carneiro explores DNA scaffolds in tissue regeneration, Dr. Prakki characterizes restorative dental materials. In each case improvements in understanding drive fundamental research, promoting highly engaging learning environment for our HQPs while supporting our interactions with industrial and research partners. 38 HQPs working with the applicants and additional numbers from other Faculty of Dentistry PIs and collaborating institutes require access to this equipment.

我们今天所知的万能机械测试系统(INSTRON)已经成为许多研究领域不可或缺的设备。自1946年第一台Instron万能应变片试验机问世以来，它已成为生物力学研究的主力，提供检查和测量生物力学响应力的能力，为研究人员开辟了数量惊人的新领域，使他们能够更好地了解和量化生物和工程系统中的应力-应变关系。其中的影响包括发现生物系统结构和功能之间的关系，以及仿生生物材料的发展。这些系统和方法的最新进展使研究人员能够可视化和测量动态事件。所要求的通用测试系统将用电脉冲E1000取代我们退役的通用测试系统。退役万能试验机是Instron 4301。它在实验室中运行了30多年，由于不可更换的部件故障不得不退役。所要求的系统将与我们在过去二十年中为适当安装样品而设计的现有夹具兼容，以便在各种生物材料上可靠地执行疲劳测试，特别是牙科植入物。该系统将设在二级生物安全实验室附近，并与牙学院先进的显微镜/成像设备集成在一起。这将是一个独特的设施，用于对体外组织移植和原位硬组织进行生物力学研究。该系统将得到我们现有用户群的支持。这一设施将允许私人投资者和共同申请者研究静态和动态生物力学事件，以获得对硬组织和合成生物材料的更好了解。Kishen博士研究三维组织移植和牙本质组织，Bozec博士研究胶原结构和生物力学特性，Changoor博士研究骨关节炎中的软骨生物力学，Manolson博士研究骨质疏松的骨生物力学，Santerre博士研究自由基生成的聚氨酯化学，它提供嵌段共聚物的化学多样性，相分离形成分子间结构的能力，从而产生影响细胞功能的物理线索，从而推动一项创新聚合物生物材料开发计划。卡内罗博士探索了DNA支架在组织再生中的作用，普拉基博士则描述了修复牙科材料的特征。在每一种情况下，理解的改善都推动了基础研究，促进了我们HQP的高度参与性学习环境，同时支持我们与工业和研究合作伙伴的互动。38名与申请者一起工作的HQP以及来自其他牙科学院PI和合作机构的额外号码需要接触到此设备。