Protein-mineral interactions at the organic-inorganic interface in biominerals

生物矿物质中有机-无机界面的蛋白质-矿物质相互作用

• 批准号: RGPIN-2022-03238
• 负责人: McKee, Marc
• 金额: $ 4.08万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=750712
• 关键词: Protein; mineral; interactions; organic; inorganic

Bones, teeth, otoconia, eggshells, snail shells, sea shells, corals, and many other biomineralized structures arise from synergistic interactions between co-existing organic (usually proteins) and inorganic mineral (often calcium-based) phases. These interactions produce hard, composite biological materials having specialized properties, largely attributable to hierarchically organized, supramolecular assemblies that provide a framework for remarkable biomineralized architectures. Negatively charged proteins within this organic framework (the extracellular matrix) may stabilize amorphous mineral precursor phases, or they may attain regulatory chemical complementarity by binding to lattice calcium at the organic-inorganic interface - mechanisms that influence crystal growth processes. It is hypothesized that the molecular precision of such organic-inorganic interfacial interactions regulates crystal growth to produce such hardened, biomineralized composite structures. My NSERC DG biomineralization research program focuses on specific biomolecules and proteins/peptides (notably osteopontin) that regulate calcium carbonate mineral growth (specifically here, the forms calcite and vaterite). Small biomolecules, amino acids, peptides and full-length proteins guide and regulate biomineralization. A common theme for calcium carbonate mineralization is that nanoparticles form within a confined, protein/peptide-rich reaction nanoenvironment, from which they crystallize and align over many length scales to build mesocrystals having occluded organics. This notion contrasts with classical crystallization theory which postulates ion-by-ion attachment. My biomineralization research program compares these scenarios by exploring fundamental principles of how relevant organics influence biomineralization. The short-term objectives for this proposal are to continue examining the extracellular regulatory mechanisms guiding Ca-carbonate biomineralization through three projects: i) reptile (gecko and snake) eggshell structure, ii) the interface (attachment) of avian and reptilian eggshell membrane fibers with the mineral of the shell, and iii) the determinants of synthetic, chiral helicoidal vaterite suprastructure growth (including simulations). Long-term objectives are to explore i) how diverse biomolecules influence Ca-carbonate growth and hierarchical assembly, and ii) how biomineral curvature (vs angulated facets) is produced in biology, particularly in shells. With new information from this research, we will understand key elements of biomineralization biology for calcium carbonate (particularly in eggshells, with implications for food safety in the case of the table egg) that might well cross over into other biomineralizing systems. Such a cross-over may potentially allow for tunable mineralization events in synthetic biomaterials with uses in tissue repair and tissue engineering applications, and for novel chiral materials with unique optical properties.

骨骼，牙齿，耳齿，蛋壳，蜗牛壳，贝壳，珊瑚和许多其他生物矿化结构是由共存有机（通常是蛋白质）和无机矿物（通常基于钙的）相之间的协同相互作用引起的。这些相互作用产生了具有专门特性的硬复合生物学材料，这在很大程度上归因于层次有组织的超分子组件，这些组件为显着的生物矿物结构提供了框架。在该有机框架（细胞外基质）内带负电荷的蛋白质可能稳定非晶矿物前体相，或者它们可以通过在有机 - 无机界面与晶格钙结合来获得调节化学互补，这会影响晶体生长过程。假设这种有机无机界面相互作用的分子精度调节了晶体生长以产生这种硬化的生物矿化复合结构。我的NSERC DG生物矿化研究计划的重点是特定的生物分子和蛋白质/肽（尤其是骨桥蛋白），该研究调节碳酸钙矿物质矿物质的生长（特别是在这里，有方解石和vathite形式）。小的生物分子，氨基酸，肽和全长蛋白指南并调节生物矿化。碳酸钙矿化的一个共同主题是，纳米颗粒在受约束的蛋白质/肽反应富含反应的纳米环境中形成，它们在许多长度尺度上结晶和对齐，以构建具有闭塞器官的中晶。该概念与假定离子依恋离子的经典结晶理论形成鲜明对比。我的生物矿化研究计划通过探讨相关有机物如何影响生物矿化的基本原理来比较这些情况。 该提案的短期目标是继续检查通过三个项目指导Ca碳酸盐生物矿化的细胞外调节机制：i）爬行动物（壁虎和蛇）蛋壳结构，ii）avian and Roptilian extrian extrian fiertiral of shelel seltrial of shemeral and iii and iii syners and iii synertal of synatiral of syners and verteral of the offian of sholuce of壳的象征（附件）（附件）（附件）。上建筑的增长（包括模拟）。长期目标是探索i）多样化的生物分子如何影响Ca-碳酸盐的生长和分层组件，ii）生物学曲率如何在生物学中，尤其是在壳中产生生物融合（VS角相）。有了这项研究的新信息，我们将了解碳酸钙生物矿化生物学的关键要素（尤其是在蛋壳中，对食品鸡蛋而言，对食品安全的影响）很可能会跨入其他生物矿化系统。这样的交叉可能有可能允许在组织修复和组织工程应用中使用的合成生物材料中可调节的矿化事件，以及具有独特光学特性的新型手性材料。