Advanced time-resolved optical sensing and imaging systems for biomedical and environmental applications

适用于生物医学和环境应用的先进时间分辨光学传感和成像系统

• 批准号: RGPIN-2019-07127
• 负责人: Fang, Qiyin
• 金额: $ 3.35万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=752915
• 关键词: Advanced; time; resolved; optical; sensing

Recent advances in photonics and electronics technologies are one of the driving forces behind many novel photonics devices development. Among these device technologies, optical sensing and imaging have been investigated for applications in life sciences research (e.g. microscopic imaging and analytical instruments), environmental sensing (e.g. water quality monitoring) and medical diagnosis (e.g. endoscopic imaging and laboratory test instruments). The majority of the instruments and devices operate by measuring the amplitude of the light intensity in the spatial (e.g. imaging) and spectral (e.g. spectroscopy) domain. Time-dependent intensity variation, especially at high speed, are not as widely used, even when many feasibility studies demonstrated that they provide important information for many applications. For example, comparing to intensive based measurements, fluorescence lifetime imaging (FLIM) can provide more efficient measure of protein-protein interaction at 1-10 nm scale, which could be very useful in drug discovery process. However, there is no commercial high content screening instrument with a high resolution FLIM capability for measuring protein-protein interaction. Another example is dissolved oxygen (DO) measurements. Although commercial frequency modulated DO instruments exist for more than 10 years, miniaturized lab-on-chip DO measurements are still relying on steady-state techniques and usually cost over $1000 each. In the proposed research program, we plan to study the integration of photonics, microelectronics, and mechanical components into next generation time-resolved optical sensors/imagers. Specifically, we will 1)develop technologies towards a FLIM microscope with the highest acquisition speed with high spatial and temporal resolution; 2)develop integrated time-resolved compact optofluidics sensor devices capable of continuous measurements while remain low-cost; 3)develop customized computational algorithms tailored to the detection technologies for accurate and fast time-domain parameter retrieval. The proposed advanced sensing and imaging technology program will be supported by the world class Micro/Nano material synthetization, component fabrication, characterization, and device integration facilities available at McMaster. The program will focus on integrated time-domain optical sensing and imaging device technology development. The applications of such technologies include life science analytical instruments, automated microscopy for drug discovery, and environmental monitoring. Research in these applications will be supported by project-based funding mechanisms including NSERC, OCE, and CIHR.

光子学和电子学技术的最新进展是许多新颖光子器件开发背后的驱动力之一。在这些设备技术中，光学传感和成像已被研究用于生命科学研究（例如显微成像和分析仪器），环境传感（例如水质监测）和医疗诊断（例如内窥镜成像和实验室测试仪器）。 大多数仪器和设备通过测量空间（例如成像）和光谱（例如光谱）域中的光强度的幅度来操作。与时间相关的强度变化，特别是在高速下，没有被广泛使用，即使许多可行性研究表明它们为许多应用提供了重要信息。例如，与基于强度的测量相比，荧光寿命成像（FLIM）可以在1-10 nm尺度下提供更有效的蛋白质-蛋白质相互作用的测量，这在药物发现过程中可能非常有用。然而，没有商业化的具有高分辨率FLIM能力的高含量筛选仪器用于测量蛋白质-蛋白质相互作用。另一个例子是溶解氧（DO）测量。虽然商业调频DO仪器存在超过10年，小型化的芯片上的实验室DO测量仍然依赖于稳态技术，通常每个成本超过1000美元。 在拟议的研究计划中，我们计划研究将光子学，微电子学和机械组件集成到下一代时间分辨光学传感器/成像器中。具体而言，我们将1）开发具有最高采集速度和高空间和时间分辨率的FLIM显微镜技术; 2）开发集成时间分辨紧凑型光流体传感器设备，能够连续测量，同时保持低成本; 3）开发针对检测技术的定制计算算法，以实现准确和快速的时域参数检索。 拟议的先进传感和成像技术计划将得到麦克马斯特世界级微/纳米材料合成、组件制造、表征和设备集成设施的支持。该计划将专注于集成时域光学传感和成像设备技术的开发。这些技术的应用包括生命科学分析仪器、用于药物发现的自动显微镜和环境监测。这些应用程序的研究将得到基于项目的资助机制的支持，包括NSERC，OCE和CIHR。