CAV1通过信号通路参与调节干细胞的生理活动。文献和我们前期研究发现表皮干细胞（ESCs）过表达CAV1能促进烧伤创面愈合，但其分子机制仍不完全清楚。我们结合研究基础推测CAV1过表达的ESCs可能通过靶向Notch信号通路调控ESCs的增殖分化促进烧伤创面的修复。本项目拟通过离体和在体模型系统的研究CAV1对Notch信号的时空表达、ESCs的增殖分化及对烧伤创面愈合的影响；基于慢病毒转染技术构建CAV1过表达的ESCs离体研究其细胞增殖、分化、黏附与迁移能力的变化，在体验证对小鼠烧伤创面愈合的影响及Notch信号的调控作用，探讨CAV1、Notch信号通路与ESCs增殖分化和烧伤创面修复的相互关系，阐明CAV1改善ESCs介导的烧伤创面修复作用的相关分子机制，为临床上促进烧伤创面修复，改善创面愈合质量开拓新的思路和提供相关理论依据。

CAV1 plays a key role in stem cells physiological activity and burn wound healing according to references and our previous study. However, the molecular mechanism is still unknown. We suppose CAV1 overexpressed in ESCs may control the differentiation of ESCs to promote burn wound healing by targeting Notch signal pathway. In this project, the effects of CAV1 in the expression of Notch signal pathway, the ESCs’ differentiation and burn wound repair will be observed both in vivo and in vitro. A lentivirus-based gene transfection technology will be performed to construct a model of ESCs that overexpresses CAV1 to study ESCs proliferation，differentiation, adhesion，migration, to illustrate the effects of CAV1 in would healing and Notch signal pathway and the mechanism of CAV1 in accelerating burn wound healing. Results may provide a new therapeutic treatment and theoretical basement to promote burn wound healing.