基于DNA组装的耦合增强SERS纳米探针用于mRNA检测及活细胞成像

表面增强拉曼散射（Surface Enhanced Raman Scattering, SERS）技术以其灵敏度高、检测速度快、谱峰窄、无损且不受自发荧光和水的干扰等优点，在分析检测和生物成像中展现出显著的优势。然而构建结构可控的、重现性好的高灵敏的SERS纳米探针仍具有挑战性。本项目拟基于DNA杂交介导的纳米组装技术，设计合成结构高度可控的二聚体、金字塔等离子体纳米探针，开展SERS增强机理研究，并以癌症高度相关的mRNA为模型，发展耦合增强SERS纳米探针的活细胞生物传感新技术；在此基础上，结合多色SERS探针构建技术，发展多种mRNA同步高灵敏检测的耦合增强 SERS生物传感技术，构建活细胞内癌症标记物高通量、高灵敏度的实时SERS成像分析新方法，为相关的生物学研究和疾病早期预防及诊断提供新的技术平台。

Surface enhanced raman scattering holds great promise for detection and biological imaging in virtue of its high sensitivity, fast detection, narrow peak, no destruction, not subject to spontaneous fluorescence and water interference. However, it has been a great challenge to construct sensitive SERS nanoprobes which are structureally controllable and reproducible. Based on DNA hybridization mediated assembly of nanoparticles，the project focuses on the development of novel dimer and pyramid coupling enhanced SERS nanoprobes which is highly controllable, and research on SERS enhancement mechanism. As well, a novel technique will be developed on coupled enhanced SERS nano-probe for live cell biosensor, which cancer-related messenger RNA as a model. Furthermore, combining with complex SERS probe construction technology, multiple target synchronization detection will be explored. A new platform will be constructed for real-time SERS imaging analysis of intracellular cancer markers with high throughput and high sensitivity.