利用经过处理的牙本质基质与牙囊干细胞构建生物牙根相关研究

利用成牙支架与牙源性种子细胞构建生物牙根有望成为修复牙缺失的新方法。目前尚未构建出具有生物活性及咬合功能的生物牙根，对构建生物牙根相关机理有待阐明。本项目组率先利用牙本质基质与牙囊细胞实现牙本质组织及生物牙根再生，同时发现Wnt通路可以对生物牙根构建过程中矿化进行有效调控。但对具体干预量及生物牙根构建策略系统性研究有待完善，对在大动物模型上进行具有咬合功能生物牙根构建有待进一步探讨。本项目拟在前期研究基础上，全面比较研究TDM与其他经典支架成牙诱导性以及牙囊细胞与其他牙源性种子细胞成牙分化能力，探讨Wnt通路对TDM诱导下牙囊细胞的分化调控，在大动物上构建生物牙根，进一步深入探索Wnt通路在生物牙根构建中可能存在调控机理，同时对生物牙根咬合功能进行分析，进而完善生物牙根构建策略。其意义在于为生物牙根临床前期实验奠定理论及生物技术基础，为临床应用提供理论指导，同时为牙根发育相关理论进行补充。

Constructing biological root (bio-root) is hopeful to become a novel strategy for treating tooth loss using odontogenic inductive scaffold and odontogenic seeding cells. However, bio-root with occlusion function still is not constructed and the mechanism is unclear. Our group firstly realized the regeneration of dentin and bio-root using treated dentin matrix (TDM) and dental follicle cells （DFCs）,found Wnt pathway could effectively regulate the mineralization during constructing bio-root. But it need to be continually explored for the suitable dosage of intervention, the systemic strategies to construct bio-root and the occlusion function of bio-root in big animal.This study, based on our previous research, comparatively investigated odontogenic capacity of between TDM and other classic scaffold regenerating tooth tissues,odontogenic differentiation between DFCs and other odontogenic seeding cells, study on Wnt pathway regulating the odontogenic differentiation of DFCs under induction of TDM, effectively construct bio-root in big aninmals and analysize the occlusion function, which optimize the strategy of constructing bio-root.The significance lie in laying theoretical foundation for pre-clinical trials for bio-root and biotechnology, provide theoretical guidance for future clinical application of bio-root and meanwhile perfect the theory relative to tooth development.