为解决分子印迹聚合物（MIP）制备时所需高交联度带来的传质慢、模板洗脱难及吸附容量低等痼疾，提出构建以其它方式保持印迹位点构型的新思路，并利用刚性棒状液晶单体形成额外物理交联网络结构的策略，首次开展低交联液晶MIP色谱固定相的研究。本课题在已成功研制低交联液晶MIP毛细管涂层手性固定相的基础上，开展多模板印迹涂层研究，结合模板单体优势构建无交联液晶MIP涂层；以基于多重引发剂的局部聚合方式制备低交联液晶MIP纳米粒；设计低交联液晶MIP接枝整体柱；并考察这些新型MIP的手性拆分能力。从传质动力学及板高分析等方面研究模板分子的传质机制；以前沿色谱、手性分离热力学及吸附模型表征其印迹位点的可接近性及不均匀性；总结聚合参数对新型MIP结构及性能影响的规律；在此基础上，实现对手性药物氢化奎尼丁、氟比洛芬、西替利嗪及尼群地平的分离。为发展高效MIP手性固定相及新一代MIP材料奠定理论和实践基础。

To solve the main problems of the slow mass transfer, the difficulty in elution and the low loading capacity of molecularly imprinted polymer (MIP), a new idea was suggested that the memory to template molecule is kept with other modes. MIP stationary phase in low crosslinking degree was prepared first with rigid liquid monomers to form additional physical crosslinking. Based on previous liquid crystal-based MIP coating in capillary, capillary coating with multi-templates imprinting, crosslinker-free imprinted coating containing templated monomer, nanoparticles prepared with multi-initiators in local polymerization, and grafted monolith MIPs will be made. Moreover, the combination of monomers containing liquid and crystal templated monomers will be used to prepare MIPs without crosslinker. The ability of chiral separation will be evaluated using the new MIPs. The mechanism of mass transfer for the templates in the MIPs will be investigated by analysis of plate height and dynamics of mass transfer. The heterogeneity and accessibility of imprinted sites will be studied by frontal chromatography, thermodynamic parameters of chiral separation and adsorptive models. The relationship between the structure and performance of liquid crystal MIPs and polymerization variables will be summarized. In addition, new method towards multi-template MIPs will be developed to separate hydroquinidine, flurbiprofen , cetirizine and nitrendipine. This work provides a new strategy to develop the highly selective and efficient MIP chiral stationary phase. Furthermore, it will contribute to a theoretical and practical foundation to the development in next generation of MIPs materials and the stationary phase of high performance.