HIV-1潜伏的转录网络与调控研究

HIV-1的潜伏是清除病毒的最后壁垒，其内在性状存在于从细胞整体到染色质局部的具体微环境中。申请者在前染色质代谢蛋白共调节HIV-1转录的研究基础上，进一步推导HIV-1潜伏主导的转录网络。具体的，以HIV-1潜伏T细胞模型为平台，以潜伏细胞全基因组转录网络化调控为主轴，挖掘以HIV潜伏为先导的miRNA-mRNA协同功能模块、 miRNA调控潜伏的路径机制化研究。系统水平上，从网络到节点，探索HIV潜伏相关的主效基因与调控机制。为开发针对HIV-1潜伏的干预措施提供疾病标记及机理依据。

HIV-1 latency is the last barrier to eradiate the infection, since the virus was integrated into the host genome, thus, affecting all the aspects of the cellular behaviour. Based on the previous study on the chromatin related regulation of HIV-1 latency, this project aims to infer and analyze the transcription network of latency. In detail, adopting the established HIV-1 latent T cell as platform, whole genome regulation of the HIV-1 latency will be disclosed by transcription network analysis. After mining HIV-1 latency directed miRNA functional module, the lean regulation of the related loop will be further explored. Therefore, at the systematic level, the project will find the hub gene and its mechanism in HIV-1 latency. Taken together, this project will uncover new diagnostic and therapeutic opportunities for purging latent HIV-1 viruses from their cellular reservoirs.