中国是世界上最大的苹果浓缩汁生产国，出口量占世界贸易量的50%以上，而苹果汁中具有致畸致癌毒性的展青霉素污染严重，生产中难以去除，成为中国苹果汁国际化发展的"绿色安全壁垒"之一。建立一种安全、高效、快速、低成本的展青霉素去除方法和技术体系是目前产业急需。本项目以近年来选育的苹果酒酵母菌株和积累的乳酸菌菌株为基本材料，甄别获得有效吸附苹果汁中展青霉素的目标微生物菌株；通过建立基于失活微生物细胞吸附展青霉素的理论模型，解析失活目标菌株对苹果汁中展青霉素的吸附结合行为，对结合位点、化学基团及化学结构进行分子表征，系统揭示失活微生物细胞吸附去除苹果汁中展青霉素的分子机制；基于分子机制并结合失活菌体细胞的饱和吸附量及处理后苹果汁品质变化的模糊综合评价优化，获得最大生物饱和吸附量的结合位点化学改性方法与失活微生物细胞固定化方法，最终构建基于失活微生物细胞的苹果汁中展青霉素去除方法体系。

China is the biggest processing and export country of apple juice concentrate (AJC) for its exports of AJC account for more than 50% in the world. However, as an important safety factor,patulin is hard to be degraded and removed completely in the process of production due to its special physical and chemical properties.Patulin residue has become one of Green trade-barriers in further internationalization development of AJC. Therefore, To establish a safe, efficient, rapid and low cost removal technique system becomes one of hot research topics and urgent needs for current industry. Target strains will be selected through screening patulin removal ability of inactivated cider yeast and lactic acid bacteria strains from our laboratory in apple juice. Patulin adsorption behaviors of inactivated cells will be analyzed primarily based on patulin adsorption theory models, and then molecular adsorption mechanisms will be revealed by molecular characterization analysis of patulin binding locus, binding chemical groups and structures. Magnetic immobilized inactivated cells will be prepared for patulin adsorption according to molecular adsorption mechanism and fuzzy comprehensive evaluation of micorbial saturated adsorption capacity and juice quality. Finally, patulin removal theories and methods using inactivated micorbial cells will be established systematically.