有机磷农药（OPs）的误用、滥用导致农产品中OPs残留超标，严重危害消费者身体健康。OPs的灵敏、快速检测技术不仅是农药残留控制研究的重要方向，也是农药检测市场的迫切需求。本项目在已结题相关检测技术研究的基础上，拟开展以下研究工作：（1）合成多种OPs的通用结构半抗原,分析通用结构半抗原与载体蛋白偶联的碳间隔臂长和偶联比对抗体特异性的影响。（2）采用细胞融合、有限稀释-ELISA技术筛选阳性杂交瘤细胞株，制备OPs的通用单克隆抗体。（3）以金纳米粒子为载体，介导辣根过氧化物酶和通用单克隆抗体，构建高灵敏信号放大探针。探讨辣根过氧化物酶和通用抗体的比例、金纳米粒子粒径与探针敏感性的关系。（4）以磁纳米微球为固相载体包被通用抗原，构建捕获探针。探讨通用抗原包被量、磁纳米微球粒径与抗原-抗体反应动力学的关系。最后基于构建的磁纳米捕获探针和金纳米信号放大探针研究OPs多残留的灵敏、快速检测技术。

The misuse and abuse of organophosphorus pesticides (OPs) could lead residual excessive of OPs which harm consumer health seriously. The technique for rapid and sensitive detection of OPs is not only the direction of scientific research on pesticide residue control but also the market demand of pesticide residue detection. Based on the previous studies on the immunoassay, this project will focus on following aspects: (1) Synthesis of a variety of OPs generic hapten. Effect analysis of carbon spacer arm length and coupling ratio between generic hapten and carrier protein to specificity of antibody. (2) Hybridoma cell selection by using cell fusion technology and limiting dilution ELISA. Production of generic monoclonal antibody (BS-McAb) specific for OPs. (3) Construction of OPs specific signal amplification probe by coating gold nanoparticles (AuNPs) with BS-McAb and horseradish peroxidase (HRP) enzyme. Relationships investigation of AuNPs diameters and the ratios between HRP and BS-McAb to the sensitivity of the probe. (4) Construction of capture probe based on magnetic nanosphere as solid support. Relationships investigation of the amount of coating antigen, magnetic nanosphere diameter to the reaction kinetics of antigen-antibody. Development of a detection technique for rapid and sensitive detection of OPs based on constructed magnetic capture probe and gold signal amplification probe.