内生真菌交织枝顶孢NRPS-ACR06587基因簇合成的生防活性多肽鉴定及其调控机制

根结线虫和枯萎病等土传病害是制约我国设施蔬菜生产可持续发展的主要生物灾害。本项目拟在前期获得主效生防活性多肽NRPS-ACR06587基因簇中生物合成酶基因敲除突变体、证明其决定生防活性产物合成作用的基础上，通过NRPS-ACR06587基因簇功能分析，生防肽类物质分离纯化与鉴定，以及生物合成基因启动子融合GUS（P06587:lacZ）和簇内转录因子启动子融合GUS(P06584：lacZ)靶标合成基因簇表达及产物合成等关键技术，揭示生防活性多肽NRPS-ACR06587基因簇的功能及其调控机制，实现从基因组到未知结构生防活性产物的种类及其分子结构解析，为土传病害绿色防控技术的创制提供理论依据和技术平台。

Soil-borne diseases such as root-knot nematode and fusarium wilt are the important biological disasters, which restrict seriously the sustainable development of vegetable production facilities. Previously, we have got the biological synthetase gene knockout mutant of NRPS-ACR06587 cluster of Acremonium implicatum, which was mainly responsible for the synthesis of biocontrol active peptides to soil-borne disease. In the proposed project, we plan to conduct the assays by analyzing the cluster NRPS-ACR06587 biological function, purifying the bioactive peptides, exploring the relationship of biosynthesis efficiency, and the synthetase gene fusion promoter (P06587:lacZ) and transcription factor fusion promoter (P06584:lacZ), to reveal the biosynthetic pathway and molecular regulatory system of biocontrol active peptides controlled by NRPS-ACR06587 gene clusters. The research would explain the series objects from the genome to bioactive peptides and its molecular structures. In addition, the results will provide a theoretical basis and technical platform on safe control technology to soil-borne disease.