甘蓝与尖孢镰刀菌粘团专化型不亲和互作的机制研究

甘蓝枯萎病是近年在我国新发生的一种毁灭性土传病害，探索甘蓝与枯萎病菌之间的不亲和互作，对于解析甘蓝抗枯萎病基因的遗传效应，培育抗枯萎病品种具有重要的意义。本项目拟在前期初步揭示甘蓝枯萎病菌的致害机制、明确品种抗性遗传规律、完成尖孢镰刀菌甘蓝专化型1号和2号生理小种的比较基因组测序和国内分离物重测序的基础上，采用基于Illumina 的RNA-seq技术，比较三种基因型甘蓝（SS，RS，RR）在1号/2号小种侵染下的全基因组转录组差异，筛选出甘蓝与尖孢镰刀菌粘团专化型不亲和互作相关的重要功能基因，并对这些基因参与抗病的作用过程进行qPRC表达动态分析；进一步对重要基因进行感病基因型甘蓝遗传转化功能分析；同时，利用同源重组敲除方法对尖孢镰刀菌粘团专化型1号/2号小种进行基因敲除功能分析。以期阐明甘蓝枯萎病菌与甘蓝互作的分子机制，为制定具有创新性的枯萎病控制及育种策略提供理论依据。

Fusarium oxysporum f. sp conglutinans (FOC), a major soil-borne fungal pathogen and one of the most destructive diseases of cabbage worldwide, causes cabbage vascular wilt disease in our country in recent years. It is significant important for the analysis on genetic effects of the cabbage resistant genes to FOC on cabbage resistant breeding. Our previous investigations have revealed the pathogenesis of FOC and the initial genetic resistance of cabbage. Moreover, we have completed the comparative genome sequencing of FOC race1 and race2. Importantly, we have finished the resequencing of pathogen collections of Chinese isolations. Based on the above results, we will plan to further conduct the following researches. Firstly, through comparing the difference transcriptomes among three genotypic cabbages (SS, RS, RR) that inoculated with race1 and race2 using RNA-Seq based on Illumina, we will screen and identify the putative important genes that might take part in the incompatible interactions between cabbage and FOC. Then we will analyze the dynamic expression of these genes during the incompatible interactions using qPCR. Next we will conduct the functional analysis by genetic transformation of these genes on susceptible cabbages. Furthermore, we will use the RNA interfering method to knockdown the expression of endogenous genes to reveal the gene functions of FOC race1 and race2. Our goal is to clarify the molecular basis of the interactions between cabbage and FOC, and the results will provide theoretical guidance for establishing innovative control methods and breeding strategy of resistant cabbages.