Cry2Ab杀虫蛋白是一种广泛应用的生物源杀虫剂，也是二代转双价基因作物中重要的杀虫蛋白。目前对其杀虫机理的研究多从受体特异性等角度进行。我们前期的研究发现钙调磷酸酶（Calcineurin, CaN）/FK506结合蛋白信号及ABCC2参与了Cry2Ab杀虫作用，但详细的分子机制尚不清楚。本申请拟采用活体RNAi、细胞转染、Western blot、点突变等生理生化和分子生物学方法，深入解析CaN如何调控下游靶蛋白（FKBP和ABCC2）的去磷酸化及受体ABCC2的磷酸化如何影响Cry2Ab对棉铃虫的毒理。该研究有利于深入探究Cry2Ab的作用机制，为减少其使用风险奠定理论基础。

Cry2Ab protein is extensively used as biological insecticide and also used as an important protein in transgenic crop. The studies concerning the mode of action of Cry2Ab mainly focus on the identification of receptors. Our previous studies have confirmed that calcineurin/FK506 binding protein signals and ABCC2 take part in Cry2Ab action. However, the detailed molecular mechanism of these molecules involved in Cry2Ab toxicology remained elusive. In present studies, the effect of CaNA/FK506 binding protein/ABCC2 on Cry2Ab toxicity will be investigated by using RNAi, cell transfection, Western Blot, point mutations, binding experiments and so on. This study will enrich the molecular mechanism of Cry2Ab action and reduce the risk of Cry2Ab application.