Suppression of Calcineurin Enhances the Toxicity of Cry1Ac to Helicoverpa armigera.
Suppression of Calcineurin Enhances the Toxicity of Cry1Ac to Helicoverpa armigera.
复制标题
DOI：
10.3389/fmicb.2021.634619
复制
发表时间：
2021
影响因子：
5.2
通讯作者：
An S
中科院分区：
文献类型：
--
作者：
Wei J;Yao X;Yang S;Liu S;Zhou S;Cen J;Liu X;Du M;Tang Q;An S
随着转Bt基因作物种植面积的扩大，昆虫对Bt杀虫蛋白的抗性迅速进化。聚合RNA干扰（RNAi）和Bt在作物中是迫切需要的，以应对害虫抗性的快速增长。理想的“金字塔”战略同时针对不同的作用途径，相互发挥协同作用。在这里，我们确定了一个脱磷酸酶，即棉铃虫钙调神经磷酸酶（HaCAN），这可能是通过调节免疫基因表达通过脱磷酸酶活性，而不是作为一个受体来增强Cry1Ac对棉铃虫的杀虫活性。值得注意的是，阻断酶活性或敲低内源性HaCAN显著促进Cry1Ac对昆虫幼虫和细胞的毒性增强。相应地，HaCAN活性的增加降低了Cry1Ac的细胞毒性，如HaCAN的异源表达所示。我们的研究结果为HaCAN基因作为RNAi和Cry1Ac作物抗棉铃虫的重要候选基因提供了可能。
Insect resistance to Bacillus thuringiensis (Bt) insecticidal proteins has rapidly evolved with the expansion of the planting area of transgenic Bt crops. Pyramiding RNA interference (RNAi) and Bt in crops is urgently needed to counter the rapid increase in pest resistance. The ideal “pyramid” strategy simultaneously targets different action pathways that exert synergetic effects on each other. Here, we identified a dephosphatase, namely, Helicoverpa armigera calcineurin (HaCAN), which might enhance the insecticidal activity of Cry1Ac against Helicoverpa armigera by regulating immune gene expression via dephosphatase activity, but not by acting as a receptor. Notably, blocking enzyme activity or knocking down endogenous HaCAN significantly promoted the enhancement in Cry1Ac toxicity to insect larvae and cells. Correspondingly, the increase in HaCAN activity reduced the cytotoxicity of Cry1Ac as shown by the heterologous expression of HaCAN. Our results provide a probable that HaCAN is an important candidate gene for pyramiding RNAi and Cry1Ac crops to control cotton bollworm.
登录
查看更多内容
影响因子：
2.2
作者：
Chen，Li-Zhen;Liang，Ge-Mei;Rector，Brian G.
通讯作者：
Rector，Brian G.
影响因子：
4.8
作者：
Caccia, S.;Astarita, F.;Pennacchio, F.
通讯作者：
Pennacchio, F.
影响因子：
3.8
作者：
Cancino-Rodezno, Angeles;Alexander, Cynthia;Villasenor, Roberto;Pacheco, Sabino;Porta, Helena;Pauchet, Yannick;Soberon, Mario;Gill, Sarjeet S.;Bravo, Alejandra
通讯作者：
Bravo, Alejandra
酪氨酸羟化酶和多巴脱羧酶的过度表达与甜菜夜蛾蛹黑色化相关。
影响因子：
4.6
作者：
Liu S;Wang M;Li X
通讯作者：
Li X
影响因子：
9.3
作者：
Furman JL;Norris CM
通讯作者：
Norris CM