白芍为中医常用补血药，有养血柔肝解郁之特色，具有补血、神经保护、抗抑郁等作用。课题组即将完成的自然基金课题证实，芍药苷的药理作用广泛，是白芍的有效但非专属成分，而研究较少的芍药内酯苷为白芍的特征有效成分，药效研究成果申报了专利。本课题旨在以白芍中医功效为指导，以芍药苷、芍药内酯苷分离纯化专利技术为支撑，在血虚肝郁证动物模型基础上，依据对相关中医证候神经-内分泌-免疫网络等方面的研究结果，引入代谢组学技术分析血虚肝郁证各代谢标志物的变化，明确其物质基础和病理特征，比较白芍（芍药苷、芍药内酯苷）的作用特点，整合血虚肝郁证与白芍及有效成分养血柔肝解郁功效的"证-效"关联性。进一步比较芍药苷、芍药内酯苷对cAMP/PKA通路CREB、BDNF等靶点影响的异同，从整体、组织、细胞、分子不同层面探讨白芍作用特点和机制，揭示物质基础，阐明中药药性理论的科学内涵，为白芍的临床应用提供科学依据。

According to the Chinese Pharmacopoeia (2010ed),Baishao, Paeoniae Radix Alba has the pharmacological effects of blood enriching and emolliating liver. Paeoniflorin have more studies on pharmacological effects but lack of the albiflorin, which cause increasingly more attention. It has confirmed the effects of blood enriching and antidepressant of albiflorin for the first time by our last NSFC project. This subject is base of the traditional efficacy as a guide in Baishao and our patents of separation and purification of paeoniflorin and albiflorin. In the last project, we had set up the animal model of the blood deficiency and depression syndrome, with the metabonomics analysis technology lead in to explicit the material and pathological features of the animal model to combined with the research progress on the Nerve-Endocrine-Immune network to analysis the relationship of syndrome and effect. Based on them, compare the difference of paeonia and albiflorin via the cAMP/PKA pathways to explore effective constituent, characteristic and mechanism of the pharmacological effects, to clarify the scientific connotation of the Traditional Chinese Medicine, to provide a scientific basis of the clinical application of Baishao.