蜂蜜花源酚类标记物与酚类对DNA氧化损伤的保护机制

酚类化合物是广泛存在于蜂蜜中的一类有机化合物，具有较高的抗氧化活性。已有研究表明，酚类化合物可以作为单花种蜂蜜的花源标记物，因而引起了研究者的关注。中国拥有较多的单花种蜂蜜，但对其花源标记物、酚类化合物的结构、抗氧化活性及抗氧化机制了解较少。本研究应用有机分析的方法，表征椴树蜜、荆条蜜及龙眼蜜中主要酚类化合物的结构；采用指纹图谱技术确定3种单花种蜂蜜花源的酚类标记物；以活性氧诱导人体外周淋巴细胞DNA和pBR322DNA损伤为模型，评价3种蜂蜜酚类化合物对活性氧致DNA氧化损伤的保护作用，探究其保护机制，从而筛选高抗氧化活性的酚类化合物。研究结果将为蜂蜜溯源及质量控制提供依据，同时为高活性天然抗氧化剂提供新的开发资源，对蜂蜜的加工利用及深入认识蜂蜜的中药药理活性具有重要意义。

Phenolic compouns ,including flavonoids and phenolic acids,are the main bioactive compoents of honey, which exhibit high antioxidant activity. Some phenolic compounds can be considered as floral markers of honeys. Linden tree honey, vervain family honey and longan honey are the main monofloral honeys of China, however, there is limited research on the structural characterization of their antioxidants and antioxidant activities. In this study, we will characterize the structure of phenolic compouns of three monofloral honeys and evaluate the protective action of these honeys and their antioxidant phenolic compounds on peripheral lymphocyte DNA and pBR322DNA damage induced by reactive oxygen species. The findings will help to understand the structure of antioxidants of three monofloral honeys and their roles in the botanical origin of chinese monofloral honey and preliminarily reveal the protective mechanisms of these honey and their antioxidants on the oxidative DNA damage and in vivo antioxidant activity. The results will provide the evidence for the floral markers of honey, and also provide the new antioxidant resource from honeys. It is also important to develop the antioxidant honey product and elucidate the pharmacological activity of honeys.