蛋白质糖基化是最为普遍和最为重要的翻译后修饰种类之一，糖基化修饰在疾病中，特别是肿瘤的发生、发展和转移过程中具有极其重要的意义，蛋白质糖基化程度及糖链结构的异常变化常常是癌症及其他疾病发生的标志，从蛋白质糖基化入手寻找生物标志物将更加特意和更加灵敏。然而，糖基化这样一个重要的翻译后修饰蛋白质组之所以尚未被广泛开展并不是因为意义不够重大，而是因为缺乏有效的大规模研究方法以及高灵敏的目标糖蛋白质检测方法。本项目针对糖蛋白质组研究中的几大关键技术问题发展相应的分析新方法，包括1)新型复合功能纳米材料高选择性分离富集糖蛋白质,2)微纳固定酶反应器高效糖蛋白质酶解和去糖链,3)化学衍生提高糖肽和糖链的离子化效率,4)同位素标记糖蛋白质的相对定量技术和MRM绝对定量技术，和5) 分子弛豫技术高灵敏、快速检测目标糖蛋白质，为挖掘重大疾病如大肠癌等的糖蛋白质生物标志物提供有效的技术方法和翔实的数据。

Glycosylation is one of the most universal and important protein post-translational modifications (PTMs), which has vital significance in disease, especially in the tumorigenesis progression and metastasis. The degree of glycosylation and changes of glycan has been identified as cancer biomarkers. However, study on such an important post-translational modifications in proteome has not been widely developed due to lack of effective methods for large scale research. Aiming at key techniques in glycoproteome field, this program will develop corresponding new analytical methods, including highly selective separation and enrichment technique for glycoprotein by novel composite-functional nanomaterials, highly effective glycoprotein digestion and deglycosylation technique by immobilized enzyme reactor, technique for enhancing ionization efficiency of glycopeptides and glycan by chemical derivation, glycoprotein relative quantification technique by isotope labeling relative quantification and MRM absolute quantification technique, and highly sensitive and fast detection method for targeted glycoprotein by intermolecular relaxation. All of these techniques contribute to the high-sensitive and high-accurate identification and high-precision quantification of glycoprotein and provide effective methods and plenty data for seeking glycoprotein biomarker in diseases such as colorectal cancer.