益生菌发酵药渣及其苷类代谢产物对脾虚小鼠的调理机制研究

药食两用中草药是我国传统中药瑰宝。其与人体肠道益生菌相互促进，可提高机体营养代谢及免疫功能。中药渣是可再生资源，我国由于缺乏药渣生物利用方面的基础研究，其大量废弃并造成环境污染。目前，益生菌高校利用药渣方面，鲜见报道。我们前期研究中，益生菌可在药渣不添加任何外来物质的前提下大量繁殖，发酵产物具有良好的DPPH、O2－和HO自由基清除能力、Fe2+螯合能力和高还原性，且可有效抑杀常见的食源性致病菌。本研究拟以健胃消食片药渣为研究对象，利用益生菌产生的多元化酶系有效释放药渣中的药效成分，将健胃消食片在健脾方面的良好疗效与益生菌本身对脾虚患者良好的恢复作用有机结合，通过实时荧光定量分析、16S rDNA高通量测序、小鼠常规特征分析、脾器官及组织的免疫学指标来阐述健胃消食片药渣发酵液及苷类物质在预防和改善脾虚症状的作用机制，为我国中药药渣的开发利用提供新思路，为丰富中药微生态学理论提供实验依据。

Medicine food homology medicine is the treasure of Chinese herbal medicine, which can enhanced the human immunization and metabolism function interacted with probiotics in human intestine. However, herb medicine residues became the big problems for environmental pollution because of the lack of the basic study on the development of medicine residues. Till now, few study is carried out to solve the environmental pollution and recycling of the medical composition in medicine residues using probiotics. Our previous studies showed that probiotics could grow well in medicine residues without any foreign substances, possessing good resistance to oxidation and bacteriostatic effect. In the present study, residues of Jianwei xiaoshi tablets was chosen to ferment using probiotics with powerful enzyme system, which combine the spleen enhancement effects of Jianwei xiaoshi residues and probiotics. Then the real-time fluorescence quantitative analysis, 16s rDNA high-throughput sequencing technology, conventional characteristics of mice, immunological indexes of spleen were used to explore the mechanism of jianwei xiaoshi fermented liquid to prevent and improve the spleen deficient mice, providing new mentality for the development and utilization of Chinese herbal medicine in our country and enriching of the experimental basis for the traditional Chinese medicine micro ecology.