胃癌严重危害人类健康，其发病机制需深入探讨，lncRNA是目前备受关注的热点之一。我们前期通过高通量筛选结合TCGA数据库分析，发现两个胃癌关键lncRNA：UCA1和LOC101928316。扩大组织样本研究发现二者不仅在胃癌早期发生明显改变，且与胃癌进展程度显著相关，细胞研究也获得同样结论，进一步结合mRNA表达谱分析和细胞预实验发现这两个lncRNA可能通过对PI3K-Akt-mTOR信号通路的调控发挥作用，但机制有待深入阐明。本研究应用细胞模型，构建UCA1和LOC101928316对PI3K-Akt-mTOR通路的调控网络，结合动物模型，阐明这两个lncRNA调控PI3K-Akt-mTOR通路在胃癌发生发展中的作用，最后通过大样本人群研究，探讨UCA1和LOC101928316及相关分子作为胃癌早期诊断、评估和预后判断生物标志的可能。研究为进一步揭示胃癌分子机制及其防治提供依据。

Gastric cancer which causes serious damage to human health, however, its pathogenesis remains unclear. The role of long non-coding RNA (lncRNA) is becoming a new hot spot in this field. Our previous study has found that two key lncRNAs—UCA1 and LOC101928316, which were related with gastric cancer by high-throughput screening and The Cancer Genome Atlas (TCGA) database analysis. Expand tissue samples study found that the two key lncRNAs not only has obviously changed in the early gastric cancer, but also significantly associated with gastric cancer progression. In addition, our cell research also gets the same conclusion. Furthermore, combining the mRNA expression profile analysis and cell experiments, we found that the two key lncRNAs may regulate the PI3K-Akt-mTOR signaling pathway, but the mechanism remains to be further clarified. In this study, we construct the UCA1 and LOC101928316 regulation network of PI3K-Akt-mTOR signaling pathway by cell experiments, and also combine the animal model experiments to clarify the two key lncRNAs roles of regulate PI3K-Akt-mTOR signaling pathway in the development of gastric cancer. Finally, we use large population-based study to further investigate UCA1 and LOC101928316 and their related molecules as biomarkers for gastric cancer early diagnosis, assessment and prognostic. The results will supply the evidence for further revealing the molecular mechanisms and provide a basis for prevention and treatment of gastric cancer.