东亚钳蝎氯毒素BmK CT是由我国科学家分离、纯化的蝎氯毒素类似物，能够特异性结合脑胶质瘤细胞上的氯离子通道和基质金属蛋白酶2，抑制胶质瘤细胞的浸润生长和迁移。本课题组前期对I-131标记BmK CT在脑胶质瘤动物模型上进行了分子影像及靶向治疗研究，结果提示131I-BmK CT能够显示荷胶质瘤动物的肿瘤病灶且明显抑制肿瘤的生长。但SPECT显像的空间分辨率有限，CT显像可提供精确的解剖信息。由于树状大分子包裹金纳米颗粒具有优异的表面修饰性、生物相容性及CT成像性，且在肿瘤组织中有增强的通透与滞留效应（EPR 效应），可作为构建肿瘤多模态分子探针的理想载体。本研究拟用I-131标记BmK CT修饰的树状大分子包裹金纳米颗粒，制成靶向胶质瘤的新型诊治一体化纳米探针，并在体内、外评价其靶向显示及治疗胶质瘤的效果，为活体内实时、无创监测胶质瘤疗效提供多模态分子影像学方法。

As an analogue of chlorotoxin, Buthus martensii Karsch chlorotoxin (BmK CT) was isolated and purified by Chinese scientists. It has been demonstrated that BmK CT can inhibited glioma cells growth and metastasis by specifically blocking the chloride ion channels and matrix metalloproteinase-2 on tumor cells. In the former work of our group, we successfully radiolabeled BmK CT with I-131. The inhibition rate of 131I-BmK CT to C6 glioma cells was significantly higher than that of unlabeled BmK CT. The in vivo imaging study showed that 131I-BmK CT accumulated in C6 tumors with good contrast. However, the spatial resolution of SPECT is limited. On the basis of previous work, we aim to combine 131I-BmK CT with dendrimer-entrapped gold nanoparticles, demonstrate its binding affinity and inhibition rate of malignant glioma cells in vitro and in vivo, and display its potential for targeted radiotherapy of glioma. Our study will provide a novel theranostic nanoprobe for non-invasive imaging and targeted radiotherapy of malignant glioma.