中药材质量的稳定性和可控性直接影响临床用药的有效和安全。滇龙胆作为中药材龙胆的基源植物之一，主产云南，目前野生资源匮乏，栽培种质混杂不清，导致药材品质不稳定。若要揭示品质差异遗传本质，对功能基因的研究是最直接和关键的手段。本项目拟在高通量转录组测序和其他研究的工作基础上的，克隆参与滇龙胆的主成分龙胆苦苷生物合成的三个候选关键酶基因(香叶醇-10 羟化酶、环烯醚萜合酶、裂环烯醚萜合酶基因)，进一步通过对候选关键酶基因采用时空表达特性以及体外酶促反应进行功能验证确定关键酶基因。在此基础上，以同生境下栽种的不同种质滇龙胆为材料，研究三年生滇龙胆关键酶基因的SNPs和CNVs与龙胆苦苷积累的关系，以揭示滇龙胆品质差异遗传本质。研究结果可为滇龙胆的品质评价提供依据，也可为品质调控奠定基础。

The stability and controllability of Chinese herbal medicines can directly influence the effectiveness and safety of clinical drug use. Gentiana rigescens，as one of the original plants of Traditional Chinese Medicine“longdan”, mainly distributed in Yunnan. At present, the wild resources were lack and cultivated germplasm was confused. To reveal the genetic essence of quality difference, research on the functional genes is the most direct and key method. On the basis of high-throughput transcriptome sequencing and some other research, the project intends to clone three candidate key enzyme genes participating in gentiopicroside biosynthesis.The three enzyme genes include geraniol 10-hydroxylase, iridoid synthase and secologania synthas. Then, the key enzyme genes were determined by functional verification. Gene functions were detected by time-space of gene expression and in vitro enzymatic. On this foundation, The study focused on correlation between key enzyme gene SNPs and CNVs and gentiopicroside synthesis of three years of Gentiana rigescens, which collecting from different germplasm were planted in the same habitat. The study aimed at uncovering the genetic essence of quality difference in Gentiana rigescen. The results can provide the basis for evaluating the quality of Gentiana rigescens, but also lay the foundation for quality control.