勃起功能障碍（ED）严重影响患者生活质量，预计2025年全球ED患者可达3亿人。研究能够修复ED病理变化和恢复自然勃起的治疗方法，是重要的科学问题。近年来低能量冲击波（LESW）在ED临床及基础研究得到广泛重视，而LESW治疗疾病的机理还不透彻。前期研究中，我们发现LESW可显著增加大鼠阴茎局部EdU阳性前体/干细胞数量和基质细胞衍生因子-1（SDF-1）表达。但是，LESW及SDF-1招募内源性干细胞治疗ED的机制还不清楚。本研究拟用骨盆神经血管损伤性ED大鼠模型，探明LESW对ED靶器官中SDF-1时空表达及体内内源性前体/干细胞动态分布的影响；应用SDF-1/CRISPRa技术提高阴茎局部SDF-1表达，探明SDF-1及其与LESW在招募体内前体/干细胞治疗ED中的协同作用机制；并在体外研究中，对上述机制进一步验证。本研究为开发安全有效的ED治疗方法和提高人类生殖健康水平有重要意义。

Erectile dysfunction (ED) seriously influence the quality of life, ED patients is expected up to 300 million people worldwide in 2025. It is important to find methods that can fix pathological changes and restore natural erection for ED patients. In recent years, low energy shock wave (LESW) has been widely investigated in clinical and basic research, though the mechanism of LESW in the treatment of disease is not clear. In the previous study, we found that LESW could significantly increase the number of EdU positive precursor/stem cells and the expression of stromal cell derived factor-1 (SDF-1) in rat penis. However, the mechanism of recruiting endogenous stem cells by LESW and SDF in treating ED is unclear. This study aims to explore the time and space expression of SDF-1 and the dynamic distribution of endogenous precursor/stem cells under the stimulation of LESW in a pelvic neurovascular injuries rat model, to investigate the synergy mechanism of recruiting endogenous stem cells by LESW and SDF in treating ED, and to further validate the above-mentioned mechanism in vitro. This research will be helpful for the development of a safe and effective treatment for ED and will improve the level of human reproductive health in future.