脂肪干细胞（ADSCs）取材简单，含量丰富，而且自体移植没有伦理学问题，已经成为临床上种子细胞的重要来源。前期工作中我们参考两步法人胚胎干细胞诱导分化NK细胞的程序，应用造血祖细胞的诱导培养基和NK细胞诱导培养基成功的将ADSCs诱导分化成NK细胞(inNK)，并且将inNK注射入前列腺癌荷瘤裸鼠体内，一个月后肿瘤体积缩小60%以上。因此本研究拟在前期工作的基础上，通过调整培养基中各种细胞因子的组合和剂量，进一步改进造血祖细胞诱导培养基，构建高效的诱导造血祖细胞生长微环境，提高ADSCs分化成造血祖细胞的成功率。然后，我们通过E4BP4转染技术和改进NK细胞诱导培养基，提高造血祖细胞分化NK细胞的速度和成熟度。通过体外实验观察诱导的NK细胞对肿瘤细胞的毒性和特异性。再将诱导的NK细胞注入前列腺癌荷瘤裸鼠体内观察其对肿瘤的杀伤作用及其对机体的影响。

When compared to other stem cells populations and sources, adipose-derived stem cell （ADSC） can be easily isolated while providing simultaneously higher yields and have not ethics issue. Previously, we have shown that ADSC can generate NK cells using a 2-step in vitro differentiation scheme and have antitumor activity in vivo. In this study,through a variety of cytokines to build hematopoietic stem cells microenvironment, we developed a cytokine-based method for ADSC-induced hematopoietic stem cell. Then, development of NK cell induction medium and transfected-E4BP4 to hematopoietic stem cell enhances NK cell ripe speeds. We further evaluated the toxicity and specificity of ADSC-induced NK cells in vivo and in vitro experiments.