棕点石斑鱼是海南近年来发展起来的养殖新品种，具有重要的经济价值和广阔的发展前景，但在海南等地的棕点石斑鱼养殖中，病害爆发频繁并严重制约了该产业的发展，为了在保障养殖产品质量的前提下有效控制棕点石斑鱼疾病的发生与蔓延，本项目拟采用本课题组建立的鱼类免疫增强剂二联筛选法研究筛选能显著提高棕点石斑鱼非特异性免疫力的中草药免疫增强剂，并在此基础上采用Solexa高通量测序技术研究投喂或注射中草药免疫增强剂后棕点石斑鱼头肾和脾脏等免疫器官中特异表达的基因信息，研究筛选中草药免疫增强剂诱发棕点石斑鱼表达的免疫应答特异分子。本项目研究不仅有助于为棕点石斑鱼爆发性流行病的控制寻找安全高效的防治药物，还有助于建立和完善渔用中草药免疫增强剂的快速高效筛选方法和了解棕点石斑鱼的免疫机制，对推动渔用中草药免疫增强剂的开发应用和棕点石斑鱼健康养殖的可持续发展具有重大意义

Epinephelus fuscoguttatus, which has high economic value and good development foreground, is a new culture species developed in Hainan province in recent years. However, diseases broke out frequently in its culture and thus seriously restricted its development. In order to control the eruption and spread of its diseases and improve the quality of its cultured products, this project firstly aims to screen Chinese herbal immunostimulants with good effects on improving the nonspecific immunity of Epinephelus fuscoguttatus by a self-developed new bi-screening method. Then, the Solex technology will be used to analyze the specific expression gene information in the head kidney and spleen of Epinephelus fuscoguttatus fed with Chinese herbal immunostimulants. Last, the key immune related genes in the head kidney and the spleen of Epinephelus fuscoguttatus stimulated by screened Chinese herbs would be confirmed. The results of this study will not only be helpful to develop safe and efficient drugs for controlling the fulminant diseases in Epinephelus fuscoguttatus, but also be helpful to establish and improve the fast screening methods for Chinese herbal immunostimulants for fish and reveal the immune mechanism of Epinephelus fuscoguttatus. Therefore, this study will play important roles in promoting the development and application of Chinese herbal immunostimulants for fish and the sustainable development of healthy culture of Epinephelus fuscoguttatus.