驼背鲈属珍稀名贵海水鱼类，具有重要的经济价值，养殖发展前景广阔。近年来，我国海南等地已突破了驼背鲈的人工繁育技术并实现了其苗种的规模化生产，本项目组也通过在饵料中添加钝顶螺旋藻和乳酸乳球菌等措施突破了驼背鲈养殖中成活率低和生长速度慢等瓶颈。然而，有关钝顶螺旋藻和乳酸乳球菌对驼背鲈免疫功能的影响及其免疫调节机制尚不清楚，对驼背鲈免疫生物学特征的研究迄今仍属空白。为此，本项目将在优化钝顶螺旋藻和乳酸乳球菌使用方案和确定其对驼背鲈非特异性免疫力影响的基础上，采用Solexa高通量测序和实时定量荧光PCR相结合技术，并借助生物信息学分析等手段确定经该2种饵料添加剂诱导后驼背鲈头肾和脾脏等主要免疫器官转录组的特异表达基因，项目成果不仅可从血清学水平和转录组水平阐明该2种饵料添加剂提高驼背鲈非特异性免疫力的作用机制，还对了解驼背鲈的免疫生物学特征和促进驼背鲈人工养殖可持续发展有重要意义。

Hamperback grouper,Cromileptes altivelis, is a rare and precious seawater fish. With high economic and ornamental values, its aquaculture prospect is bright. In recent years, its artificial breeding technique has been broken through and large sum of fry could be obtained in Hainan. Our research group has successfully improved the survival rate and growth rate of hamperback grouper by adding Spirulina platensis and Lactococcus lactis in its feed. However, the effect of Spirulina platensis and Lactococcus lactis on its immunity and its genetic regulation mechanism was still not clear, and the immunology character of hamperback grouper is blank either. In this project, the operation scheme of Spirulina platensis and Lactococcus lactis will be optimized and the effect of these two feed addatives on the nonspecific immunity of hamperback grouper will be studied. Then, the specific expression genes of the main immuno-related organs (headkidney and spleen) transcripts after being induced by Spirulina platensis and Lactococcus lactis will be identified by Solexa high-throughput sequencing technique, Real-Time PCR and bioinformatics analysis. The results of this study will not only explore the function mechanisms of these two immunostimulants’ effect on improving the nonspecific immunity of hamperback grouper from serological and transcriptomic level, but also be helpful for understanding the immunological characteristcs of hamperback grouper and promoting the sustainable development of its culture.