项目针对设施蔬菜栽培中土壤盐渍化日益严重，导致作物光合效率下降，生长发育受抑制，生产效益下降的问题，以设施栽培的重要蔬菜作物黄瓜为试材，采用营养液栽培，研究外源腐胺调控盐胁迫下黄瓜幼苗类囊体膜蛋白适应性变化的作用机制。采用蓝绿温和胶电泳技术对黄瓜类囊体膜蛋白进行分离，结合质谱鉴定技术，研究外源腐胺对盐胁迫下类囊体膜蛋白组表达的影响，明确差异表达和特异合成蛋白的种类及性质；利用RT-PCR技术，对应答膜蛋白进行基因表达分析，研究差异膜蛋白表达及其编码基因在转录和翻译水平上的相关性；测定叶绿体内不同形态多胺含量和转谷酰胺酶（TGase）活性的变化，设计、构建TGase单克隆抗体，结合免疫分析和免疫金-透射电镜观察技术，探讨外源腐胺介导TGase与类囊体膜蛋白的互作效应。旨在从蛋白和基因水平，阐述外源腐胺缓解盐胁迫对植株光合作用抑制的分子生物学机制，为利用多胺提高设施蔬菜抗盐性提供理论依据。

As the soil salinization in protected cultivation was serious, often leading to photosynthesis inhibition and limiting plant growth, the present study was conducted in nutrient solution to investigate the mechanism of exogenous Put on adaptive changes of thylakoid membrane proteins in cucumber seedlings under salt stress. Blue native polyacrylamide gel electrophoresis was used to separate the thylakoid membrane proteins, combined with mass spectrometry identification technique, to explore the effects of exogenous Put on the expression of thylakoid membrane proteins, and identify the type and property of specific and differently expressed proteins. RT-PCR was applicated to gene expression analysis of response membrane proteins, in order to show the relationship of differently expressed proteins and encoding gene in the levels of transcription and translation. We determinated polyamines forms and TGase activity in chloroplasts, and designed and synthesized TGase monoclonal antibody. Combined with the immunoassay and immuno-gold-transmission electron microscope techniques,the work aimed to investigate the exogenous Put-mediated interplay of TGase and thylakoid membrane proteins. The objective of present study was to elaborate the molecular biological mechanism of Put in alleviating the photosynthesis inhibition induced by salt stress, which would provide a theoretical support for the improved salt tolerance of protected vegetable by polyamines.