发酵乳制品是乳酸菌理想的递送载体，如何保证乳酸菌存活和益生性，是乳品工业要解决的关键问题。乳酸菌对乳成份的附着力决定了它们在乳基质中的定位，并影响乳酸菌生长和发酵乳成熟，但乳酸菌与乳成份的互作效应尚未完全探明。申请人通过前期研究和文献查阅关注到乳酸菌有趋向附着乳脂肪球膜（MFGM）特性，且MFGM蛋白高度糖基化、并有与肠道类似的黏附素受体。申请人设想“乳酸菌与MFGM蛋白可能存在互作效应，且MFGM蛋白可能会介导乳酸菌生长代谢、保护及黏附特性”。因此，本项目拟在了解乳酸菌趋向附着MFGM基础上，探讨乳酸菌与MFGM蛋白互作效应及机制，利用MFGM蛋白构建乳酸菌微胶囊载体，探索MFGM蛋白对乳酸菌的保护效应，并进一步探讨MFGM蛋白介导乳酸菌黏附定植行为。本项目的实施，可进一步了解乳酸菌与乳基质成分互作，为提高乳酸菌的存活率和黏附特性提供新思路；并为乳酸菌与MFGM蛋白产品开发提供理论基础。

Fermented dairy products are the ideal delivery carrier of lactic acid bacteria (LAB). How to protect survival and beneficial growth of LAB is the key problem to be solved in the dairy industry. The adhesion of LAB to milk components determines their localization in the matrix, which affects the growth of LAB and the maturation of fermented milk. Through previous studies and literature review, the applicant noticed that LAB tended to attach to milk fat globule membrane (MFGM). The MFGM protein is highly glycosylated and has an adhesin receptor similar to that of the intestinal tract. The applicant assumes that "LAB may be interacted with MFGM protein, and MFGM protein may mediate the growth, metabolism, protection and adhesion characteristics of LAB". Therefore, based on the understanding of the tendency of LAB to adhere to MFGM, the project explored the interaction effect and mechanism between LAB and MFGM protein. After constructing the microcapsule carrier of LAB using MFGM protein, the protective effect of MFGM protein on LAB was explored. The project further explored that effect of the MFGM protein mediated the adhesion and colonization of LAB. The implementation of this project can further understand that the interaction effect with the milk matrix components; provide new ideas for improving the survival rate and adhesion characteristics of LAB. It also provides a theoretical basis for the development of dairy product containing LAB and MFGM protein.