核酸化学修饰偶联和光交联新技术在非编码RNA研究中的应用

人类基因组中编码蛋白质的基因只占不到2%，至今发现至少80%人类基因具有生物学功能，而多达90%的基因组DNA转录为RNA，其中绝大多数为非编码RNA。研究表明，非编码RNA几乎参与调节生命的所有过程，并且许多重大疾病与非编码RNA的异常表达有关,但至今对非编码RNA的发现和研究仅局限于冰山一角，迫切需要突破性的新技术和新方法应用于非编码RNA研究。本项目基于“光交联”、“点击”化学、锁核酸和靶向修饰等四项核酸关键技术，开发非编码RNA (CLIP、CLIP-seq、RIP-seq)、蛋白与非编码RNA相互作用、非编码RNA特别是lncRNA定位、功能等研究新方法，为非编码RNA研究提供有力工具。

Up to now, it has found only 2% protein-coding genes in the human genome, but 80% human genes have a biological function, and up to 90% DNA of the genome transcribed into RNA, majority of which are non-coding RNAs. Studies have shown that non-coding RNA involved in regulating the almost all life processes, as well as the abnormal expression of non-coding RNA is associated with many major diseases. But so far the functions and roles of non-coding RNA just is the tip of iceberg, desperately needs new tools and technologies for the breakthrough research of non-coding RNA. This project is based on the core technologies of "photo-crosslinking", "Click" chemistry, locked nucleic chemistry (LNA) and nucleic acid conjugation chemistry,to develop methods of non-coding RNA research tools (such as CLIP, CLIP-seq and RIP-seq), the interactions of non-coding RNA and RNA-binding proteins, non-coding RNA localization and functions in cell.