基于分子识别诱导聚集发光的典型卡西酮类毒品传感器的研究

本项目将样品净化能力突出的电膜萃取(EME)、特异性富集能力强的分子印迹聚合物(MIPs)与聚集诱导发光(AIE)效应联用用于开发卡西酮类毒品快速检测的新方法。该基于AIE的荧光法克服了传统荧光法利用荧光猝灭原理来检测目标物的不足之处，从原理上提高了荧光法的检测灵敏度，因此本课题是一种源头创新。项目合成可聚合的AIE单体，以卡西酮类毒品为模板分子，采用表面分子印迹技术制备荧光分子印迹聚合物传感器(AIE-MIPs)。优化AIE-MIPs的制备方法、分子识别性能及荧光性能之后，针对实际样品，采用不同方案将EME与AIE-MIPs联用，选择EME与AIE-MIPs高度匹配的实验条件。采用毒品检测“金标准”液质法对EME/AIE-MIPs联用法进行验证，探索其实际应用的可行性。本项目的研究将从原理上为制备便携式荧光毒品检测设备提供支持，在法医毒品快检方面具有良好的应用前景。

In this project, the clean-up capability of electromembrane extraction (EME), the specific recognition of molecularly imprinted polymers (MIPs) and the aggregation-induced–emission (AIE) will be integrated for rapid determination of cathinones. Fluorescence method is one of the most popular methods for rapid on-site determination of illicit drugs. However, the sensitivity of traditional fluorescence probe is limited mainly due to fluorescence quenching and matrix effects. Therefore, the application of AIE for the detection of cathinones is an academic innovation. The sensor of molecularly imprinted polymers with AIE property (AIE-MIPs) are prepared via surface imprinting with AIE monomers, which are synthesized based on the properties of the target (e.g. cathinone, mephedrone, ethcathinone or flephedrone). Subsequently, the preparation method, the selectivity and AIE property of AIE-MIPs are optimized, and the combination between EME and AIE-MIPs is investigated by different ways to select the optimal operation condition for EME/AIE-MIPs. This EME/AIE-MIPs approach is validated with blood, urine and saliva samples, and the applicability of EME/AIE-MIPs is evaluated using Gold Standard method (LC-MS). We believe this EME/AIE-MIPs approach will provide accurate, sensitive and rapid determination of trace target drugs in complex samples. Nevertheless, the study of the current project will provide theoretical support for the construction of portable fluorescent equipment for rapid on-site determination of drugs in the field of forensic science.