本项目针对癌症无法早期诊断重大难题，基于近红外光生物不吸收，不激发生物荧光的性质，以低能近红外上转换高能近红外发光的纳米生物探针为主线，结合固相生物“柔性”修饰技术，借助倏逝波特异性激发荧光生物标记探针和光纤传感生物信息手段，构建上转换发光生物探针基光纤传感器和建立在体血液检测新方法。开展提高UNBP上转换效率的新途径及其相关科学基础问题、固相表面生物“柔性”修饰及其相互作用动力学、UNBP基自参考量化EWOFBS制备及多重性能的研究以及EWOFBS结构对在体血液流速的影响及其与检测灵敏度和特异性的关系研究。将重点解决离子高浓度掺杂UNBP纳米新结构与近红外上转换效率的关系、固相表面与耦联抗体分子相互作用对其灵敏度和特异性的影响规律以及体内高速流动血液与EWOFBS相互用及其对检测灵敏度和特异性影响的三个关键科学问题，为实现癌症等重大疾病在体早期诊断和预警提供创新技术平台和物理基础。

In this project, the new bio optical sensor and its immunoassay method will be studied based on optical fiber and upcoversion luminescence bio-probes for saving the major difficulties with realizing early diagnosis of cancer etc. by combining no adsorption of near infrared light by biomolecules, no excitation of self biofluorescence, upconversion bioprobes from near infrared light to near infrared light, the technology of “soft” biomodification of nanoparticle surface, evanescent wave characteristics and biosensor of optical fiber. To study: (1) these based problems with enhancing the upconversion luminescence efficiency; (2) the biotechnology of “soft” modification of nanoparticle surface and the dynamics of interaction between the biomolecules and the surface of nanoparticles; (3) the preparation of biosensor based bio fiber with self-reference quantitative biodetection and upcoversion luminescence bioprobe; (4) effects of the structure of bio sensor of optical fiber based on upconversion bioprobes on in vivo blood flow velocity, biodetection sensitivity and specificity. . The key problems to be solved in this proposal include (1) the fundamental scientific approaches to increase upconversion luminescence, (2) effects of the interaction between upconversion nanoparticles and biomolecules on sensitivity and biospecificity, (3) effects of in vivo blood flow velocity and the interaction between the biosensor and blood flow on sensitivity and specificity of the quantitative immunoassay for getting a technical platform and physical foundation.