高原睡眠障碍是全身组织急速缺氧的复杂进展式睡眠障碍，后期会恶化形成高原肺水肿和高原脑水肿，危及生命。然而其分子机制尚未阐明。本课题将基于传统中医的气血理论，深入探究补血汤药当归补血汤是否对高原睡眠障碍有调控作用。课题组前期研究发现当归补血汤可以舒缓血管，降低胞内钙离子浓度，缓解急性低氧诱发的症状。项目将以黑腹果蝇为模型，模拟高原低氧状态，利用多组学联用阐明高原睡眠障碍的发病分子机制；再借助分子对接模拟技术从当归补血汤小分子库中筛选有效分子，利用体外蛋白微量热泳动亲和力实验与体内果蝇相关生理生化指标检测，找到汤药中最有效的抗缺氧小分子（对）。从现代生物学角度阐释益气养血、活血化瘀治疗高原睡眠障碍的理论，为基于气血理论找到筛选新的联合靶向小分子药物提供实验依据。

High Altitude Sleep Disorders (HASD) is a complex progressive disease which the body is suffered in acute hypoxia condition. It may lead to life-threatening high-altitude pulmonary edema (HAPE) and high-altitude cerebral edema (HACE). However, the molecular mechanism has not been elucidated. This study will explore the mechanism of Danggui Buxue Decoction (DBD) for treatment of HASD based on TCM theory of Qi-Blood.The previous work suggested DBD could reverse vascular contraction, decrease intracellular calcium concentration, in order to alleviate AMS symptoms. This study will use Drosophila Melanogaster as a model, Multiomics as a tool to find the molecular mechanism of HASD; then, the molecular docking simulation technology will be used to select the effect molecules of DBD database, while the molecules will be verified by MicroScale Thermophoresis (MST) in vitro and physiological biochemical values in vivo. This study will explain the therapeutic mechanism of “Invigorate Qi and Nourish Blood” to treat AMS, and provide the experimental evidence to investigate new combined target molecular drugs.