百合是世界范围内的著名球根类观赏植物和经济作物。体细胞胚发生是最有希望解决百合种球培育、种质保存和遗传性状改良等问题的重要再生途径。目前植物体细胞胚的发生机制尚未得到全面阐述。本研究在明确百合体细胞胚起源方式、胚性细胞出现的时间节点，并初步证实生长素对体细胞胚性程序启动的先决作用的基础上，聚焦于体细胞胚发生初期体细胞胚性获得这一关键环节，以胚性细胞出现的早期标志WUSCHEL蛋白的同源框蛋白WOX为切入点，揭示WOX在生长素介导百合胚性细胞形成中的关键作用；进一步通过探究生长素信号通路上关键调控因子ARF蛋白与目标WOX间可能的互作关系，试阐释在生长素诱导百合胚性细胞形成中ARF蛋白对WOX的上游调控作用。研究结果将为系统深入研究百合体细胞胚发生的分子机制奠定理论基础，并为其他植物的体细胞胚发生研究提供研究思路。

Lily is the famous bulbous ornamental plants and economic crops in the world. Somatic embryogenesis is the most promising regeneration pathway to solve problems of bulb production, germplasm preservation and genetic improvement in Lilium. So far, the mechanism of somatic embryogenesis in various plants has not been fully elucidated. In our previous work, the origin style of somatic embryogenesis, the time node at which embryonic cells appear had already been clarified, and the essential role of auxin on the initiation of somatic embryogenic process was preliminary confirmed in Lilium pumilum. In this study, we focus on the embryogenic property acquisition of somatic cell at the very early stage of embryogenesis and take the WOX protein, the homeobox protein of WUSCHEL usually been regarded as early marker of the appearance of embryonic cells, as the study entry point, trying to reveal the key role of WOX in embryogenic cell formation of lily induced by auxin. Further, the possible interaction between ARF protein, the key regulatory factor in auxin signaling pathway, and target WOX will be explored, thus may reveal the upstream regulation of ARF protein on WOX in lily embryogenic cell formation induced by auxin. The results of this study will lay a theoretical foundation for the systematic study of the molecular mechanism about somatic embryogenesis in Lilium and provide research ideas for the study of somatic embryogenesis in other plants.