High-efficiency somatic embryogenesis techniques for different hybrids of cut lilies
High-efficiency somatic embryogenesis techniques for different hybrids of cut lilies
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切花百合不同杂交种的高效体细胞胚胎发生技术
DOI：
10.1007/s11240-020-01904-4
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发表时间：
2020-08
期刊：
影响因子：
--
通讯作者：
Sun Hongmei
中科院分区：
文献类型：
--
作者：
Yan Rui;Wang Chunxia;Wang Jinxia;Nie Ruxia;Sun Hongmei
体细胞胚胎发生（SE）是一种独特的离体再生方法，用于植物繁殖、种质资源保存和分子辅助育种。百合是一种具有国际价值的观赏植物，但其SE技术尚不完善。以5个百合品种为材料，研究了不同外植体、毒莠定（PIC）浓度和光照条件对百合体细胞胚诱导、发育、萌发和成苗的影响。对百合胚的组织形态、发育和起源进行了观察，首次建立了百合胚状体发生体系。以叶片为外植体，所有供试品种均进行了间接体细胞胚胎发生（SE）。以亚洲百合'草莓奶油'、东方百合'双惊喜'和麝香百合为试材，以鳞片为外植体，进行离体培养。“白色天堂”进行间接SE，麝香百合×亚洲百合“眼线”和麝香百合×东方百合“凯旋”进行直接SE。上述品种的体细胞胚胎发生经历球形胚、心形胚、鱼雷形胚和子叶胚四个时期。两个原始SE途径（内源性和外源性）进行了观察，其出现时间不同。在间接SE中，先出现外源胚，然后是内源胚;而在直接SE中，顺序相反。体细胞胚来源的不同以及内外来源比例的不同，通过直接或间接途径与体细胞胚的发生密切相关，并显著影响体细胞胚的诱导周期。暗培养促进了百合体细胞胚的诱导，而16 h/d光照培养促进了百合体细胞胚的萌发，5个百合品种的萌发率均超过90%。MS+（1.5-2.5 mg L−1）PIC + 0.2 mg L−11-萘乙酸（NAA）培养基对百合叶片和鳞片的SE诱导效果最好，诱导率为88- 96%。
Somatic embryogenesis (SE) is a unique method of in vitro regeneration for plant reproduction, germplasm resource preservation, and molecular-assisted breeding. Although lily is an internationally valued ornamental plant, its SE technology is still incomplete. Here, the effects of different explants, picloram (PIC) concentrations, and light conditions on somatic embryo induction, development, germination and seedling formation were studied in fiveLiliumvarieties belonging to different hybrids. The embryo histomorphology, development and origins were observed, and a broadly efficient embryogenesis system was firstly established inLilium. With leaf explants, all varieties tested underwent indirect somatic embryogenesis (SE). With scale explants, theLiliumAsiatic ‘Strawberry and Cream’,LiliumOriental ‘Double Surprise’, andLilium longiflorumThunb. ‘White Heaven’ underwent indirect SE and theLilium longiflorum× Asiatic hybrid ‘Eyeliner’ andLilium longiflorum× Oriental ‘Triumphator’ underwent direct SE. Somatic embryogenesis in the above varieties involved four stages: globular, heart-shaped, torpedo-shaped, and cotyledon embryos. Two original SE pathways (endogenous and exogenous) were observed, and their appearance times differed. In indirect SE, exogenous embryos appeared first, followed by endogenous embryos; whereas in direct SE, the order was reversed. The different somatic embryo origins and the proportion of internal and external origins were closely related to the occurrence of somatic embryos by direct or indirect pathways and significantly affected the induction cycle of somatic embryos. Dark culture promoted lily somatic embryo induction, while 16 h/day light culture promoted germination, and the germination rates of all five lily varieties exceeded 90%. Murashige Skoog (MS) medium + (1.5–2.5 mg L−1) PIC + 0.2 mg L−11-Naphthaleneacetic acid (NAA) was suitable for SE from lily leaves and scales with an induction efficiency of 88–96%.
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影响因子：
5.4
作者：
A. Jäger;S. P. Mohoto;F. V. van Heerden;A. Viljoen
通讯作者：
A. Jäger;S. P. Mohoto;F. V. van Heerden;A. Viljoen
影响因子：
1.5
作者：
Nandhakumar Natarajan;Sathish Sundararajan;S. Ramalingam;Perinba Suresh Chellakan
通讯作者：
Nandhakumar Natarajan;Sathish Sundararajan;S. Ramalingam;Perinba Suresh Chellakan
DOI：
10.1007/s11240-009-9577-z
发表时间：
2009-08
期刊：
Plant Cell, Tissue and Organ Culture (PCTOC)
影响因子：
--
作者：
P. Perera;V. Vidhanaarachchi;T. Gunathilake;D. Yakandawala;V. Hocher;J. Verdeil;L. Weerakoon
通讯作者：
P. Perera;V. Vidhanaarachchi;T. Gunathilake;D. Yakandawala;V. Hocher;J. Verdeil;L. Weerakoon
DOI：
10.1007/s11240-006-9137-8
发表时间：
2006-08
期刊：
Plant Cell, Tissue and Organ Culture
影响因子：
--
作者：
Tolga Yildirim;Z. Kaya;K. Isik
通讯作者：
Tolga Yildirim;Z. Kaya;K. Isik
影响因子：
6.9
作者：
Filonova, LH;Bozhkov, PV;von Arnold, S
通讯作者：
von Arnold, S