体细胞胚发生对于百合种质保存、遗传性状改良、种球繁育及植株发育等的基因调控均具有重要意义，而miRNA作为调控因子在胚胎发生过程中起重要作用，但miRNA调控植物体细胞胚发生的模式和分子机制尚不明确。本研究在已建立细叶百合和兰州百合体细胞胚发生体系并初步证实百合体细胞胚发生可能存在特异miRNA调控网络的基础上，选取胚性转化、胚性保持和体胚发育3个关键阶段的7类典型培养物，通过Illumina测序结合RT-PCR分析鉴定关键miRNA，并采用qRT-PCR验证其表达模式；再以生物信息学方法预测miRNA对应靶基因，并通过表达谱分析揭示其差异表达情况；最后利用miRNA转基因过表达及5'RACE技术，探索百合体细胞胚发生过程中关键miRNA和靶基因的功能及其调控机理，以期为深入挖掘体细胞胚发生的关键基因奠定基础，并为最终明确百合体细胞胚发生的分子机制、实现体细胞胚发生的人工调控提供理论依据。

Somatic embryogenesis is vital to the germplasm preservation, genetic traits improvement and gene regulation for bulb propagation as well as plant development in Lilium. And miRNA plays an important role as a transcription regulator in the somatic embryogenesis. However, the regulation pattern and molecular mechanisms of miRNA remain largely unknown as yet. On the basis of our previous work that establishing the somatic embryogenesis system in Lilium pumilum and Lilium davidii var. unicolor, we have initially confirmed that there was some specific regulatory network in somatic embryogenesis on Lilium. In this project, seven kinds of typical culture during embryogenic transformation, embryogenic keep as well as embryogenesis were adopted. Firstly,we plan to combine Illumina sequencing with RT-PCR to identify the key miRNA, and use qRT-PCR to verify its expression pattern. Then based on the bioinformatics predict the corresponding target genes, and through the expression profile analysis show the expression differences. Finally,using the miRNA transgenic overexpression and 5'RACE technology, to explore the function and regulation mechanism of key miRNAs and the target genes in the process of somatic embryogenesis of Lily.The targets of the experiments are digging the key genes in somatic embryogenesis and lay a foundation for making clear the molecular mechanism of somatic embryos in lily, furthemore, providing theoretical and practical basis for realizing the artificial regulation of somatic embryogenesis.