EgTOE1调控草原龙胆可育性重瓣花形成机制研究

草原龙胆具有典型的单轮又有多轮花器官，花器官是重要的园艺观赏性状，尤其是花瓣的轮数往往作为园艺观赏植物的育种目标。本研究团队多年来致力于草原龙胆资源收集、纯系制备及品种选育研究，前期利用遗传分析方法，通过草原龙胆单、重瓣的品种杂交获得F1代，自交获得F2代群体，利用RAD测序技术Mapping到单、重瓣花差异基因EgTOE1，初步分析重瓣花为EgTOE1+获得性突变体。本项目将利用转基因及基因编辑技术研究EgTOE1基因的功能，利用ChIP-qPCR及qRT-PCR技术筛选EgTOE1调控的特异的B、C类基因及明确在单、重瓣花中调控的模式的差异，利用IP-MS技术在单、重瓣花中筛选与EgTOE1相互作用的差异蛋白。最终明确基部合瓣花多轮花瓣形成且可育的机理，其结果将对园艺植物重瓣花新品种的育种奠定理论基础。

The number of petals in a flower is one of the most important appearance qualities of ornamental flowers. In Eustoma grandiflorum, the number of petals fluctuates significantly and little is known about how it is controlled. Our team has collected many different Eustoma grandiflorum cultivars with diverse characteristics in flower shape, color and size. In previous work, we have found that the EgTOE1 may participate in double flower formation by using the genetic analysis and map based cloning approach in segregating populations derived from a cross of single flower cultivar with and double flower cultivar. The EgTOE1 in double flower cultivar is a gain of function mutation in contrast to the single flower cultivar. In this work, we will figure out the mechanisms of EgTOE1 controlling double flower formation in Eustoma grandiflorum by a series of studies, such as: gene over-expression and gene editing by genetic transformation; analyze the expression pattern of genes related to flower development by ChIP-qPCR and qRT-PCR; screening the EgTOE1 interacted proteins by IP-MS. Our findings will provide novel insights into the molecular mechanisms of Double flower formation with fertilizability in Eustoma grandiflorum and also be very helpful for breeding new Double flowers cultivars in ornamental plant.