Aft基因型番茄LA1996为紫色花青素积累品系，利用红光、远红光、蓝光、UV-A、UV-B、蓝光+UV-B及UV-A+UV-B等不同波长单色光及复合光照射果实，发现仅在蓝光+UV-B复合光照射时诱导果实花青素合成，表明番茄LA1996存在蓝光+UV-B协同效应诱导的光受体及信号因子参与调控。本研究以Aft基因型番茄LA1996野生型为试材，利用EMS诱导获得光照下无花青素合成的突变体，通过表型观测、基因表达检测、后代群体分离分析等多重筛选，筛选出稳定遗传的目标突变体，并与野生型回交构建BC1F2分离群体。通过突变表型群体的高通量测序分析、突变候选基因与突变性状的遗传连锁分析，确定目标突变基因，并对突变基因的功能预测分析。最终发现、分离介导蓝光+UV-B复合协同特异诱导花青素合成途径中的光受体与光信号转导因子，为明确新的蓝光+UV-B光信号调控花青素合成机制奠定基础。

Plants exposed to UV exhibit protective responses including elevated anthocyanin biosynthesis. A tomato accession LA1996 with Anthocyanin fruit (Aft) gene accumulates anthocyanins in fruits. In our previous study, we found that anthocyanin accumulation in this fruits was induced only by co-irradiation with UV-B and blue but not by other wavelength lights including red, far-red, blue, UV-A or UVA+UV-B. UV-B + blue induction of anthocyanin biosynthesis is commonly observed in plants, but its signaling pathway is poorly understood. To understand this pathway, we constructed EMS mutated lines of LA1996, and screened for mutants that lost UV-B + blue induced anthocyanin accumulation. These mutants are expected to be defective in one of the components of UV-B + blue signaling pathway, or in aft genes. The purpose of this study is to identify the mutated genes of these mutants and find new key members of UV-B + blue signaling pathway that regulate anthocyanin biosynthesis.