作为由我国科学家发起和组织的"国际黄瓜基因组计划" 已完成了黄瓜全基因组的测序，获得了黄瓜基因组框架图，目前基因组序列精细图谱的绘制已基本完成。然而，在最新的序列图谱上，已锚定到染色体上的序列还远远小于黄瓜基因组的大小，在每条染色体的重叠群上都有多个缺口存在。本项目拟用荧光原位杂交技术分析黄瓜已组装序列在每条染色体上的分布情况和覆盖比例，构建黄瓜高分辨率的分子细胞遗传图谱，并与黄瓜的近缘种进行比较荧光原位杂交作图。该研究不仅有助于黄瓜基因组序列精细图谱的进一步完善，而且将对阐明黄瓜属系统分类现存问题和物种的起源进化关系提供重要信息，并为深入开展黄瓜属物种间的远缘杂交和跨物种之间克隆基因提供重要的理论依据。

The draft genome sequence of Cucumis sativus var. sativus L. were obtained by "International cucumber genome project". Fine assembly of genome sequence has basically completed. However, the total length of the assembled genome sequence is much smaller than the cucumber genome size. There are many gaps among contigs on each chromosome. Here, distribution and coverage ratio of the assembled sequences on chromosomes will be analyzed and the molecular cytogenetic maps of cucumber chromosomes will be constructed by fluorescence in situ hybridization (FISH). Furthermore, the comparative maps will also be developped by FISH mapping of cucumber fosmid clones onto the chromosomes of cucumber close relatives. This will facilitate fine assembly of genome sequence, phylogenetic relationship study of Cucumis, distant hybridization breeding and cross-species gene cloning in cucumber with close relatives.