微生物介导的肠道碳水化合物代谢对早期断奶仔猪的肠道健康及饲料高效利用至关重要，本课题组前期结果表明长×大二元杂交猪肠道内微生物介导的碳水化合物消化代谢能力显著弱于从江香猪，然将从江香猪仔猪的粪便微生物移植到长×大二元杂交猪肠道内可显著增强肠道微生物介导的碳水化合物代谢的能力。本项目拟进一步利用中国地方猪从江香猪资源，基于无菌猪、粪便微生物移植、微生物多组学等方法，全面发掘调控早期断奶仔猪肠道内碳水化合物代谢的关键差异微生物，结合体外和体内试验，验证其调控早期断奶仔猪肠道碳水化合物代谢的功能；进而运用微生物全基因组测序、转录组测序和蛋白质组学方法全面筛选出关键差异微生物的碳水化合物代谢相关酶，综合运用基因敲除和生物化学等方法阐明关键差异肠道微生物调控碳水化合物代谢的机制。研究结果旨为肠道菌群干预改善早期断奶仔猪肠道健康和提高仔猪对日粮中碳水化合物利用效率提供理论依据与新资料。

Microbes-mediated intestinal carbohydrates metabolism is vital for improving intestinal health and feed efficiency in early weaned piglets. Our previous study revealed that crossbred piglets (Landrace×Yorkshire) have poor abilities of microbes-mediated intestinal carbohydrates digestion and metabolism than that in Congjiang miniature piglets. Fecal microbiota transplantation (FMT) from the Congjiang miniature piglets into crossbred piglets (Landrace×Yorkshire) recipients significantly enhanced the ability of microbes-mediated intestinal carbohydrates digestion and metabolism. The aim of this project is to further systematically screen the key microbes mediated intestinal carbohydrates metabolism in early weaned piglets from the Congjiang miniature pigs resources via germ-free pigs model, FMT, and microbial multiple omics analyses, and to validate the key microbes-mediated intestinal carbohydrates metabolism in vitro and in vivo. Then we further systematically screen key enzymes associated with microbes-mediated intestinal carbohydrates metabolism via microbial whole genome sequencing, transcriptome sequencing, and comparative proteomics, and elucidate the mechanism of the key microbes-mediated intestinal carbohydrates metabolism through gene knockout and biochemical assays. These results may provide the valuable data for improving intestinal health and feed efficiency in early weaned piglets by the manipulation of gut microbiota.